PPP1R16B
PPP1R16B (protein phosphatase 1 regulatory subunit 16B, also known as TIMAP, KIAA0823 and ANKRD4) is an ankyrin repeat protein that regulates protein phosphatase 1 (PP1). It acts as a positive regulator of pulmonary endothelial cell barrier function. Browse Assay Genie's current range of PPP1R16B research reagents below, or read on for the biology behind the target.
PPP1R16B is a regulator of protein phosphatase 1 (PP1) that acts as a positive regulator of pulmonary endothelial cell (EC) barrier function. It is involved in regulating the PI3K/AKT signalling pathway, angiogenesis and endothelial cell proliferation, and it controls angiogenesis and proliferation through effects on ECE1 dephosphorylation, trafficking and activity. It also contributes to the regulation of endothelial cell filopodia extension.
In barrier biology, PPP1R16B protects the endothelial barrier from lipopolysaccharide (LPS)-induced vascular leakage. It is involved in PKA-mediated dephosphorylation of moesin, which is important for barrier protection against thrombin stimulation. It promotes the interaction of PPP1CA with RPSA/LAMR1, facilitating dephosphorylation of RPSA/LAMR1, and is involved in the dephosphorylation of EEF1A1. Researchers studying the PPP1R16B protein therefore often use it as a model for how a targeting subunit directs PP1 to particular substrates.
The PPP1R16B gene encodes a membrane-associated protein with five ankyrin repeats, a PP1-interacting domain and a carboxy-terminal CAAX box domain. The CAAX box may allow the protein to bind to membranes, where it may act as a signalling molecule through its interaction with PP1. Synthesis of the protein is inhibited by transforming growth factor beta-1, and it may be a downstream target of the TGF-beta1 signalling cascade in endothelial cells. Alternative splicing gives multiple transcript variants encoding different isoforms. HGNC places it in the ankyrin repeat domain containing, PP1 regulatory subunit and myosin phosphatase targeting families; UniProt records it at the cell membrane, nucleus and cell projections.
PPP1R16B positively regulates pulmonary endothelial barrier function and protects against LPS-induced vascular leakage and thrombin-related barrier disruption.
It regulates angiogenesis and endothelial cell proliferation through control of ECE1 dephosphorylation, trafficking and activity, and is involved in PI3K/AKT signalling.
PPP1R16B promotes PPP1CA interaction with RPSA/LAMR1 and contributes to dephosphorylation of RPSA/LAMR1, moesin and EEF1A1.
Synthesis of the protein is inhibited by TGF-beta1, and it may be a downstream target of this pathway in endothelial cells.
PPP1R16B is a regulator of protein phosphatase 1 that positively regulates pulmonary endothelial cell barrier function. It is involved in PI3K/AKT signalling, angiogenesis, endothelial cell proliferation and filopodia extension, and it helps direct PP1 towards substrates such as moesin, RPSA/LAMR1 and EEF1A1.
PPP1R16B is also known as TIMAP, KIAA0823 and ANKRD4. Previous names include protein phosphatase 1, regulatory (inhibitor) subunit 16B. NCBI notes that alternative splicing produces multiple transcript variants encoding different isoforms of the protein.
A mouse ortholog, Ppp1r16b, is recorded for the human gene, so rodent models can be used to study its function. Antibody and ELISA kit users should check the datasheet of any product for its validated reactivity before choosing a species.
Protein phosphatase 1 catalytic and regulatory subunits, plus related phosphatase family members, with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q96T49; product specifications from Assay Genie datasheets.