TSN
TSN (translin; also TRSLN, BCLF-1 and REHF-1) is a DNA-binding protein that recognises sequences at chromosomal translocation breakpoint junctions. It also shows endoribonuclease activity and is linked to activation of the RNA-induced silencing complex (RISC). Browse Assay Genie's current range of TSN research reagents below, or read on for the biology behind the target.
TSN has two described activities. As a nucleic acid-binding protein, translin specifically recognises conserved target sequences at the breakpoint junction of chromosomal translocations. Translin polypeptides assemble into a multimeric structure, and this assembly is responsible for its DNA-binding activity. TSN also exhibits both single-stranded and double-stranded endoribonuclease activity.
In RNA silencing, TSN may act as an activator of the RNA-induced silencing complex (RISC) by facilitating endonucleolytic cleavage of the siRNA passenger strand. When complexed with translin-associated protein X, translin forms a Mg ion-dependent endoribonuclease that promotes RISC activation. UniProt places the protein in the translin family and records it in both the cytoplasm and the nucleus.
Recombination-associated motifs and translin-binding sites are present at recombination hotspots and may serve as indicators of breakpoints in genes that are fused by translocations. These binding activities may play a crucial role in chromosomal translocation in lymphoid neoplasms. The gene, which has a mouse ortholog named Tsn, undergoes alternative splicing that gives rise to multiple transcript variants. Researchers studying the TSN gene or the translin protein will find it relevant to both genome stability and small RNA biology.
Translin binds conserved sequences at translocation breakpoint junctions, making TSN a point of reference for work on recombination hotspots and gene fusions.
Translin-binding activities may contribute to chromosomal translocation in lymphoid neoplasms, so TSN is studied in relation to breakpoint formation.
TSN may activate RISC by promoting cleavage of the siRNA passenger strand, which is relevant to studies of RNA silencing mechanisms.
TSN cleaves single- and double-stranded RNA, and with translin-associated protein X forms a Mg ion-dependent endoribonuclease complex.
TSN binds DNA at conserved sequences found at chromosomal translocation breakpoint junctions, and it has endoribonuclease activity against single- and double-stranded RNA. It may also activate RISC by helping to cleave the siRNA passenger strand.
TSN is the HGNC symbol for translin. Aliases and previous symbols include TRSLN, BCLF-1 and REHF-1. The gene undergoes alternative splicing, so multiple transcript variants are reported. Check the datasheet of any product for its validated reactivity.
Complexed with translin-associated protein X, translin forms a Mg ion-dependent endoribonuclease that promotes RISC activation. Its DNA-binding at breakpoint hotspots may play a role in chromosomal translocation in lymphoid neoplasms. The mouse ortholog is Tsn.
Translin partners and other RNA silencing components that may be of interest alongside TSN.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q15631; product specifications from Assay Genie datasheets.