COL4A3
COL4A3 (collagen type IV alpha 3 chain, formerly collagen, type IV, alpha 3 or Goodpasture antigen) encodes the alpha 3 subunit of type IV collagen, a major structural component of basement membranes. Its C-terminal NC1 domain also gives rise to tumstatin. Browse Assay Genie's current range of COL4A3 research reagents below, or read on for the biology behind the target.
Type IV collagen is the major structural component of basement membranes and is a multimeric protein built from three alpha subunits. Six genes encode alpha 1 through alpha 6, and each subunit can form a triple helix with two other subunits. The COL4A3 gene encodes alpha 3, and the resulting collagen alpha-3(IV) chain is secreted into the extracellular matrix, where it sits in the basement membrane.
The protein is also a source of a bioactive fragment. Tumstatin, a cleavage fragment corresponding to the collagen alpha 3(IV) NC1 domain, has both anti-angiogenic and anti-tumour cell activity. UniProt notes that these two properties may be regulated through RGD-independent, ITGB3-mediated mechanisms. The protein belongs to the type IV collagen family, which HGNC places in the network forming collagens gene group.
COL4A3 was previously named collagen, type IV, alpha 3 (Goodpasture antigen) because of its role in Goodpasture syndrome. In this condition, autoantibodies bind collagen molecules in the basement membranes of alveoli and glomeruli, and the epitopes lie largely in the non-collagenous C-terminal domain. A specific kinase phosphorylates amino acids in this same region, and its expression is upregulated during pathogenesis. The COL4A3 gene is also linked to an autosomal recessive form of Alport syndrome, with the contributing mutations located in the exons encoding the C-terminal region. Like other type IV collagen genes, it is organised head-to-head with another type IV collagen gene, and each gene pair shares a common promoter.
COL4A3 encodes a subunit of type IV collagen, the major structural component of basement membranes, making it relevant to studies of matrix assembly.
The C-terminal non-collagenous domain carries the main epitopes for autoantibodies binding alveolar and glomerular basement membranes in Goodpasture syndrome.
The COL4A3 gene is linked to an autosomal recessive form of Alport syndrome, with mutations located in exons encoding the C-terminal region.
Tumstatin, derived from the alpha 3(IV) NC1 domain, has anti-angiogenic and anti-tumour cell activity, possibly through ITGB3-mediated mechanisms.
COL4A3 encodes the alpha 3 subunit of type IV collagen, the major structural component of basement membranes. Three alpha subunits form a triple helix. Its NC1 domain can be cleaved to release tumstatin, which has anti-angiogenic and anti-tumour cell activity according to UniProt.
The previous HGNC name was collagen, type IV, alpha 3 (Goodpasture antigen). In Goodpasture syndrome, autoantibodies bind collagen in alveolar and glomerular basement membranes, with epitopes largely in the non-collagenous C-terminal domain of this protein.
COL4A3 is linked to an autosomal recessive form of Alport syndrome, with mutations in exons encoding the C-terminal region. It is also the Goodpasture autoantigen. The mouse ortholog is Col4a3; check the datasheet of any product for its validated reactivity.
Other collagen family members and extracellular matrix proteins with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q01955; product specifications from Assay Genie datasheets.