TAF1A
TAF1A (TATA-box binding protein associated factor, RNA polymerase I subunit A, also known as TAFI48 and SL1) is a component of the SL1/TIF-IB selectivity factor complex. It contributes to assembly of the pre-initiation complex during RNA polymerase I-dependent transcription in the nucleolus. Browse Assay Genie's current range of TAF1A research reagents below, or read on for the biology behind the target.
TAF1A is a subunit of the transcription factor SL1/TIF-IB complex, also called Selectivity Factor I. This complex takes part in assembly of the pre-initiation complex (PIC) during RNA polymerase I-dependent transcription. The rate of PIC formation is probably set mainly by how quickly SL1/TIF-IB associates with the rDNA promoter. SL1/TIF-IB also helps stabilise nucleolar transcription factor 1 (UBTF) on rDNA.
The TAF1A protein is a TATA box-binding protein-associated factor and localises to the nucleolus, the site of ribosomal RNA gene transcription. A notable feature of the complex is that formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits. This separates the TBP pool used for RNA polymerase I transcription from that used by TFIID. Researchers studying the TAF1A gene or protein are usually interested in how ribosomal DNA transcription is initiated and regulated.
The TAF1A gene is alternatively spliced, giving multiple transcript variants that encode multiple isoforms. The gene symbol is TAF1A, and the protein has also been named TAFI48 and SL1. Earlier HGNC names described it as TATA box binding protein (TBP)-associated factor, RNA polymerase I, A, 48kD. Mouse and rat both carry a Taf1a ortholog, so the target can be studied in common rodent models.
TAF1A is part of SL1/TIF-IB, which helps assemble the pre-initiation complex for RNA polymerase I-dependent transcription.
The rate of pre-initiation complex formation probably depends mainly on how fast SL1/TIF-IB associates with the rDNA promoter.
SL1/TIF-IB stabilises nucleolar transcription factor 1 (UBTF) on rDNA, making TAF1A relevant to nucleolar complex assembly.
Formation of SL1/TIF-IB excludes TBP association with TFIID subunits, which is relevant to how TBP-containing complexes are distinguished.
TAF1A is a component of the SL1/TIF-IB complex, which is involved in assembling the pre-initiation complex for RNA polymerase I-dependent transcription. SL1/TIF-IB also helps stabilise UBTF on rDNA. The protein is found in the nucleolus.
TAF1A is also known as TAFI48 and SL1. Its earlier HGNC name referred to a 48kD TBP-associated factor for RNA polymerase I. NCBI Gene reports that alternate splicing gives multiple transcript variants encoding multiple isoforms.
Yes. Mouse and rat both have an ortholog named Taf1a, so the target can be studied in rodent systems. Check the datasheet of any product for its validated reactivity.
Other TBP-associated factors and related transcription machinery targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q15573; product specifications from Assay Genie datasheets.