NAA20
NAA20 (N-alpha-acetyltransferase 20, also known as NAT5, NAT3 and dJ1002M8.1) is the catalytic subunit of the NatB complex. It acetylates the N-terminal methionine of selected proteins and supports actomyosin structure and cell migration. Browse Assay Genie's current range of NAA20 research reagents below, or read on for the biology behind the target.
NAA20 is the catalytic subunit of the NatB complex. NatB catalyses acetylation of the N-terminal methionine residue of peptides that begin with Met-Asp, Met-Glu, Met-Asn or Met-Gln. In human cells, NatB carries out this N(alpha)-terminal acetylation cotranslationally, as the nascent chain emerges, and the NCBI Gene summary specifically notes methionine residues followed by asparagine. The NAA20 protein therefore determines which newly synthesised proteins receive this modification.
Proteins with cell cycle functions are overrepresented among NatB substrates, which links NAA20 activity to cell cycle control. NAA20 is also required for maintaining the structure and function of actomyosin fibres and for proper cellular migration. The protein is found in both the cytoplasm and the nucleus. It belongs to the acetyltransferase family, ARD1 subfamily, and the HGNC places the NAA20 gene in the groups of N-alpha-acetyltransferase subunits and GCN5-related N-acetyltransferases.
The NAA20 gene has carried several names. It was previously called N-acetyltransferase 5 (NAT5), with descriptors referring to the ARD1 homologue from S. cerevisiae, and it is also listed as NAT3 and dJ1002M8.1. The current symbol follows the systematic N-alpha-acetyltransferase nomenclature. The mouse ortholog is Naa20. Researchers studying NAA20 protein levels, NatB activity or N-terminal acetylation often examine it alongside other N-alpha-acetyltransferase subunits.
NAA20 is the catalytic subunit of NatB, which acetylates N-terminal methionine on substrates beginning Met-Asp, Met-Glu, Met-Asn or Met-Gln.
Proteins with cell cycle functions are overrepresented among NatB substrates, making NAA20 relevant to studies of cell cycle control.
NAA20 is required for maintaining the structure and function of actomyosin fibres, linking N-terminal acetylation to the cytoskeleton.
NAA20 is needed for proper cellular migration, so it is a useful target for work on motility and its enzymatic control.
NAA20 is the catalytic subunit of the NatB complex. It acetylates the N-terminal methionine of peptides beginning Met-Asp, Met-Glu, Met-Asn or Met-Gln. It is also required for actomyosin fibre structure and function and for proper cellular migration.
NAA20 is also known as NAT5, NAT3 and dJ1002M8.1. Earlier names include N-acetyltransferase 5 and N(alpha)-acetyltransferase 20, NatB catalytic subunit. Older names referred to the S. cerevisiae ARD1 (arrest-defective 1) homologue, reflecting its place in the ARD1 subfamily.
A mouse ortholog, Naa20, is recorded, and the protein belongs to the ARD1 subfamily of the acetyltransferase family. Historic names also refer to an S. cerevisiae ARD1 homologue. Check the datasheet of any product for its validated reactivity.
Related enzymes and pathway partners relevant to N-terminal acetylation research.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P61599; product specifications from Assay Genie datasheets.