NEK9
NEK9 (NIMA related kinase 9, also known as Nek8 or NERCC) is a serine/threonine protein kinase of the NIMA family. It regulates mitotic progression, including spindle dynamics and chromosome separation, and activates other NEK family kinases. Browse Assay Genie's current range of NEK9 research reagents below, or read on for the biology behind the target.
NEK9 is described in UniProt as a pleiotropic regulator of mitotic progression. It takes part in the control of spindle dynamics and chromosome separation, and it is also important for the G1/S transition and S phase progression. The NCBI Gene summary notes that the protein is activated in mitosis and in turn activates other family members during mitosis, and that it mediates cellular processes essential for interphase progression.
As a kinase, NEK9 phosphorylates a range of substrates. These include several histones, myelin basic protein, beta-casein and BICD2. It phosphorylates histone H3 on serine and threonine residues, and beta-casein on serine residues. NEK9 also phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively, which places it upstream of these related kinases. UniProt records the protein in the cytoplasm and the nucleus.
The NEK9 gene encodes a member of the protein kinase superfamily, in the NEK Ser/Thr protein kinase family and NIMA subfamily. HGNC places it in the NIMA related kinase family, and its previous name was NIMA (never in mitosis gene a)-related kinase 9. Other aliases include Nek8, NERCC and NERCC1. A mouse ortholog, Nek9, is recorded. Researchers studying cell cycle control, mitotic spindle regulation or NEK kinase cascades commonly work with the NEK9 protein.
NEK9 helps control spindle dynamics and chromosome separation, making it a relevant target for studying how cells progress through mitosis.
NEK9 is important for the G1/S transition and S phase progression, and also mediates processes essential for interphase.
NEK9 phosphorylates NEK6 and NEK7 and relieves their autoinhibition, so it is useful for dissecting NEK kinase activation cascades.
NEK9 phosphorylates histone H3, myelin basic protein, beta-casein and BICD2, providing defined substrates for kinase activity work.
NEK9 is a serine/threonine kinase that regulates mitotic progression, including spindle dynamics and chromosome separation. It is also important for G1/S transition and S phase progression, and it phosphorylates NEK6 and NEK7 to stimulate their activity. Check the datasheet of any product for its validated reactivity.
HGNC lists the name as NIMA related kinase 9, previously NIMA (never in mitosis gene a)-related kinase 9. Aliases include Nek8, NERCC, NERCC1, DKFZp434D0935 and MGC16714. UniProt names the protein serine/threonine-protein kinase Nek9, and its enzyme classification is EC 2.7.11.38.
NEK9 belongs to the protein kinase superfamily, in the NEK Ser/Thr protein kinase family and NIMA subfamily. It is a member of the NIMA (never in mitosis A) kinases, and a mouse ortholog, Nek9, is recorded in the gene data.
Other NIMA related kinases, including the NEK6 and NEK7 kinases that NEK9 activates, with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q8TD19; product specifications from Assay Genie datasheets.