PRKD2
PRKD2 (protein kinase D2, also known as PKD2, HSPC187 and DKFZP586E0820) is a serine/threonine kinase of the protein kinase D family. It converts transient diacylglycerol signals into prolonged cellular effects downstream of PKC. Browse Assay Genie's current range of PRKD2 research reagents below, or read on for the biology behind the target.
PRKD2 is a serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC. It has been linked to regulation of cell proliferation through MAPK1/3 (ERK1/2) signalling, oxidative stress-induced NF-kappa-B activation, and inhibition of HDAC7 transcriptional repression. It also acts in T-cell antigen receptor (TCR) signalling and cytokine production, and contributes to Golgi membrane trafficking, angiogenesis, secretory granule release and cell adhesion.
In the trans-Golgi network (TGN), PRKD2 binds DAG and regulates the fission of transport vesicles bound for the plasma membrane. In polarised cells it is involved in transporting proteins from the TGN to the basolateral membrane, and it is required for release of chromogranin-A-containing secretory granules. In T cells, PRKD2 is activated on TCR stimulation, moves from cytoplasm to nucleus and is required for IL2 promoter up-regulation and for production of IL2 and IFNG. LCK interacts with and activates it, leading to NFAT activation. In endothelial cells it supports proliferation and migration before angiogenesis, partly by modulating KDR/VEGFR2 and FGFR1 expression, and it phosphorylates CIB1 isoform 2 to drive VEGFA secretion.
Several upstream inputs are described. Oxidative stress leads to ABL1 phosphorylation of PRKD2 and NF-kappa-B activation. Gastrin signalling through the CCKBR receptor leads to phosphorylation by CSNK1D and CSNK1E, nuclear translocation and HDAC7 phosphorylation. In epithelial cells, lysophosphatidic acid triggers a PKC-dependent pathway that drives IL8 secretion. PRKD2 belongs to the CAMK Ser/Thr protein kinase family, PKD subfamily, and can be activated by phorbol esters. The PRKD2 gene is a pleckstrin homology domain containing and microRNA host gene, and alternative splicing produces multiple transcript variants encoding different isoforms.
PRKD2 is required downstream of the TCR for IL2 and IFNG production and for optimal antibody responses to antigens.
PRKD2 supports endothelial proliferation and migration and influences VEGFA secretion, making it relevant to angiogenesis and tumour growth.
PRKD2 binds DAG in the trans-Golgi network and regulates vesicle fission and basolateral protein transport.
PRKD2 mediates oxidative stress-induced NF-kappa-B activation and LPA-stimulated IL8 secretion in epithelial cells.
PRKD2 is a DAG-responsive serine/threonine kinase acting downstream of PKC. It regulates ERK1/2 signalling, NF-kappa-B activation, HDAC7 repression, T-cell cytokine production, Golgi vesicle fission, secretory granule release and angiogenesis. Check the datasheet of any product for its validated reactivity.
PRKD2 is the HGNC symbol for protein kinase D2. Aliases include PKD2, HSPC187 and DKFZP586E0820, and UniProt names it serine/threonine-protein kinase D2. NCBI Gene notes that alternative splicing yields multiple transcript variants encoding different isoforms.
PRKD2 has mouse and rat orthologs, both named Prkd2. It belongs to the PKD subfamily of the CAMK Ser/Thr protein kinase family, which in the HGNC classification is the protein kinase D family that also includes PRKD1.
Other protein kinase D family members and kinases with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9BZL6; product specifications from Assay Genie datasheets.