RGS5
RGS5 (regulator of G protein signalling 5) is a member of the regulators of G-protein signalling gene group. It inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, switching them into their inactive GDP-bound form. Browse Assay Genie's current range of RGS5 research reagents below, or read on for the biology behind the target.
RGS5 acts as a negative regulator of G protein signalling. According to UniProt, it inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, which drives them into their inactive GDP-bound form. By accelerating this step, the RGS5 protein shortens the period in which a G protein alpha subunit remains active, and so limits the signal passed downstream from the receptor.
The protein shows selectivity in the alpha subunits it engages. RGS5 binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha, so its inhibitory activity is directed at a defined subset of G protein signalling. This selectivity is one reason researchers study individual regulators of G-protein signalling rather than treating the group as interchangeable. UniProt records the protein as 181 amino acids long and places it in the cytoplasm and at the membrane, a distribution consistent with a regulator that acts on membrane-associated G proteins.
The human RGS5 gene lies at 1q23.3, and mouse and rat each carry an orthologous gene named Rgs5, which supports comparative work across model systems. HGNC lists the approved name as regulator of G protein signalling 5, with earlier spellings of the name as regulator of G-protein signalling 5 and regulator of G-protein signalling 5. NCBI Gene also describes a locus of naturally occurring readthrough transcription between the neighbouring LOC127814295 gene and the RGS5 gene on chromosome 1. Some variants of that readthrough transcript encode novel proteins with unique N-termini, which is worth bearing in mind when designing assays that target a specific transcript or protein form.
RGS5 increases the GTPase activity of G protein alpha subunits, making it a useful subject for studying how signalling is switched off.
Because RGS5 binds G(i)-alpha and G(o)-alpha but not G(s)-alpha, it is relevant to work on selective regulation of these pathways.
As part of the regulators of G-protein signalling gene group, RGS5 is a point of comparison with other RGS proteins.
NCBI describes readthrough transcription involving RGS5, with variants encoding proteins with unique N-termini, relevant to transcript-level study.
RGS5 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, driving them into their inactive GDP-bound form. It binds G(i)-alpha and G(o)-alpha, but not G(s)-alpha. UniProt places it in the cytoplasm and at the membrane.
No aliases are listed for the RGS5 gene. HGNC gives the name regulator of G protein signalling 5, and records earlier forms with the spellings G-protein signalling and G-protein signalling. It belongs to the regulators of G-protein signalling gene group.
Yes. Mouse and rat each have an ortholog named Rgs5, so the gene can be studied in common model species. NCBI also notes a readthrough locus involving RGS5; check the datasheet of any product for its validated reactivity.
Other regulators of G-protein signalling that share the RGS gene group with RGS5.
Gene and protein annotations from HGNC, NCBI Gene and UniProt O15539; product specifications from Assay Genie datasheets.