CIP2A
CIP2A (cellular inhibitor of PP2A, also known as KIAA1524) is a protein that inhibits the serine/threonine phosphatase PP2A. It promotes MYC stability in human malignancies and works with TOPBP1 to tether broken chromosome fragments during mitosis. Browse Assay Genie's current range of CIP2A research reagents below, or read on for the biology behind the target.
CIP2A acts as an inhibitor of protein phosphatase PP2A. By preventing dephosphorylation of MYC, it stabilises MYC in human malignancies, and in this way it promotes anchorage-independent cell growth and tumour formation. Gene annotation also records protein homodimerisation activity alongside protein phosphatase inhibitor activity.
A second role concerns genome instability. Micronuclei, which are frequently found in cancer cells, consist of chromatin surrounded by their own nuclear membrane. When the micronuclear envelope breaks down, a process associated with chromothripsis, acentric chromosome fragments derived from shattered chromosomes are generated. Together with TOPBP1, CIP2A is essential for the response to this damage. The CIP2A-TOPBP1 complex tethers the fragments during mitosis so that they segregate together into a single daughter cell nucleus. This favours re-ligation with limited chromosome scattering and loss. The annotation describes the gene as involved in broken chromosome clustering.
The CIP2A protein belongs to the CIP2A family and is located in the cytoplasm (cytosol) and on chromosomes. The CIP2A gene sits within the HGNC gene group of armadillo-like helical domain containing proteins. It was previously named KIAA1524 and, before that, cell proliferation regulating inhibitor of protein phosphatase 2A. The mouse ortholog is Cip2a.
CIP2A stabilises MYC by blocking its dephosphorylation and promotes anchorage-independent growth and tumour formation in human malignancies.
As an inhibitor of protein phosphatase PP2A, CIP2A is a tool for studying how phosphatase activity controls substrate phosphorylation.
With TOPBP1, CIP2A responds to acentric chromosome fragments arising from shattered chromosomes within micronuclei.
The CIP2A-TOPBP1 complex tethers chromosome fragments in mitosis, supporting clustered segregation into one daughter nucleus and re-ligation.
CIP2A inhibits protein phosphatase PP2A and, by preventing MYC dephosphorylation, stabilises MYC in human malignancies. With TOPBP1, it also tethers chromosome fragments during mitosis after micronuclear envelope breakdown, so that the fragments segregate to a single daughter cell nucleus.
CIP2A stands for cellular inhibitor of PP2A, and UniProt lists the protein as Protein CIP2A. Its previous symbol is KIAA1524, and an earlier name was cell proliferation regulating inhibitor of protein phosphatase 2A. Check the datasheet of any product for its validated reactivity.
A mouse ortholog, Cip2a, is recorded. CIP2A is linked to cancer biology: it promotes tumour formation by stabilising MYC in human malignancies, and it acts in the response to chromosome fragments from micronuclei, which are frequent in cancer cells.
Phosphatase, MYC and DNA damage response targets connected to CIP2A biology.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q8TCG1; product specifications from Assay Genie datasheets.