FUCA1
FUCA1 (alpha-L-fucosidase 1, also known as tissue alpha-L-fucosidase) is a lysosomal glycosidase that removes fucose from the carbohydrate chains of glycoproteins. It contributes to the degradation of fucose-containing glycoproteins and glycolipids, and mutations in the FUCA1 gene are linked to fucosidosis. Browse Assay Genie's current range of FUCA1 research reagents below, or read on for the biology behind the target.
FUCA1 encodes tissue alpha-L-fucosidase, a hydrolase responsible for cleaving the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. This step is part of the lysosomal breakdown of fucose-containing glycoproteins and glycolipids, in which the enzyme releases fucose residues so that the remaining glycan structure can be processed further.
The FUCA1 protein is a lysosomal enzyme and belongs to the glycosyl hydrolase 29 family. It carries the EC number 3.2.1.51 and is classed by HGNC in the alpha-L-fucosidases gene group. The human gene has orthologues in mouse and rat, both named Fuca1, which allows the enzyme to be studied in rodent models. A pseudogene of this locus is present on chromosome 2, a point worth remembering when designing nucleic acid-based assays for the FUCA1 gene.
Mutations in FUCA1 are associated with fucosidosis (FUCA1D), an autosomal recessive lysosomal storage disease. This makes the enzyme a reference point for work on lysosomal glycan turnover and glycosidase deficiency. The gene was previously named fucosidase, alpha-L- 1, tissue. A second human fucosidase, FUCA2, is a separate gene and is a useful comparator when examining fucose-processing enzymes.
FUCA1 mutations are associated with fucosidosis, an autosomal recessive lysosomal storage disease, so the enzyme is central to studies of this disorder.
FUCA1 hydrolyses alpha-1,6-linked fucose from the reducing-end N-acetylglucosamine of glycoproteins, making it a key enzyme for studying glycan degradation.
The enzyme takes part in lysosomal degradation of fucose-containing glycoproteins and glycolipids, relevant to work on how cells recycle complex carbohydrates.
As a glycosyl hydrolase 29 family member in the alpha-L-fucosidase group, FUCA1 supports comparison with related fucosidases such as FUCA2.
FUCA1 is a lysosomal enzyme that hydrolyses the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of glycoprotein carbohydrate moieties. It takes part in the degradation of fucose-containing glycoproteins and glycolipids within the lysosome.
UniProt names the protein tissue alpha-L-fucosidase, and HGNC gives the full name alpha-L-fucosidase 1. The gene was previously called fucosidase, alpha-L- 1, tissue. No alternative gene symbols are listed. The enzyme is classified as EC 3.2.1.51.
Mutations in FUCA1 are associated with fucosidosis (FUCA1D), an autosomal recessive lysosomal storage disease. Mouse and rat both have a Fuca1 orthologue; check the datasheet of any product for its validated reactivity.
Other fucosidases and related lysosomal glycosidases with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P04066; product specifications from Assay Genie datasheets.