ZFP36L2
ZFP36L2 (ZFP36 like 2 zinc finger CCCH-type; also ERF2, TIS11D and BRF2) is a zinc-finger RNA-binding protein that destabilises AU-rich element-containing mRNAs by promoting their deadenylation. It links cellular signalling to the mRNA decay machinery and so attenuates protein synthesis. Browse Assay Genie's current range of ZFP36L2 research reagents below, or read on for the biology behind the target.
ZFP36L2 binds the 3'-untranslated region AU-rich elements (AREs) of numerous mRNAs and acts as an adapter that communicates signalling events to the mRNA decay machinery. It recruits the CCR4-NOT deadenylase complex, and probably other components of the cytoplasmic RNA decay machinery, to the bound transcripts. This promotes poly(A) tail removal and decay, providing a mechanism for attenuating protein synthesis.
The protein is annotated in UniProt as mRNA decay activator protein ZFP36L2 and belongs to the HGNC group of CCCH-type zinc fingers. Its distinguishing feature is a zinc finger domain with a repeating cys-his motif. It is found in the nucleus and the cytoplasm. The ZFP36L2 gene is a member of the TIS11 family of early response genes, which are induced by agonists such as the phorbol ester TPA and the polypeptide mitogen EGF. NCBI Gene notes that the protein most likely functions in regulating the response to growth factors. The mouse ortholog is Zfp36l2. Earlier names include ZFP36 ring finger protein like 2 and butyrate response factor 2 (EGF-response factor 2).
Reported targets and roles are varied. ZFP36L2 promotes ARE-mediated decay of LDLR mRNA after PMA treatment in a p38 MAPK-dependent manner, and of immediate early gene transcripts during early adipogenesis. It also acts on REST mRNA in mature peripheral neurons and on LHCGR mRNA in ovulation and oocyte meiotic maturation. It negatively regulates erythroid differentiation, supporting glucocorticoid-induced self-renewal of erythroid cells. Together with ZFP36L1, it maintains quiescence in developing B lymphocytes, supports accurate VDJ recombination, and is needed for thymocyte development. It also helps prevent T-ALL transformation by promoting decay of NOTCH1 mRNA.
ZFP36L2 recruits the CCR4-NOT deadenylase complex to AU-rich mRNAs, making it a model adapter for studying ARE-mediated deadenylation and decay.
With ZFP36L1, it maintains B-cell quiescence, supports VDJ recombination and thymocyte development, and restrains T-ALL transformation through NOTCH1 mRNA decay.
It negatively regulates erythroid differentiation by degrading mRNAs induced during terminal differentiation, promoting glucocorticoid-induced self-renewal of erythroid cells.
ZFP36L2 influences LDLR mRNA decay, early adipogenesis, neuronal integrity through REST, and ovulation through LHCGR mRNA decay.
ZFP36L2 is a zinc-finger RNA-binding protein that binds AU-rich elements in the 3'-UTR of mRNAs. It recruits the CCR4-NOT deadenylase complex, promoting poly(A) tail removal and mRNA decay, which attenuates protein synthesis. Targets include LDLR, REST, LHCGR and NOTCH1 mRNAs.
ZFP36L2 is also called ERF2, TIS11D and BRF2. Earlier names include ZFP36 ring finger protein like 2, butyrate response factor 2 (EGF-response factor 2) and zinc finger protein 36, C3H type-like 2. UniProt lists it as mRNA decay activator protein ZFP36L2.
ZFP36L2 belongs to the TIS11 family of early response genes, which includes ZFP36L1. The two proteins act together in developing B lymphocytes and thymocytes, promoting ARE-mediated decay of cell cycle regulator and NOTCH1 mRNAs. The mouse ortholog is Zfp36l2; check the datasheet of any product for its validated reactivity.
Other TIS11 family members and zinc finger proteins involved in post-transcriptional regulation, with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P47974; product specifications from Assay Genie datasheets.