RNF40
RNF40 (ring finger protein 40) is a nuclear RING finger E3 ubiquitin-protein ligase of the BRE1 family, also known as BRE1B, RBP95, STARING and KIAA0661. It has been linked to RB1 interaction, syntaxin 1 turnover and the KSHV lytic cycle. Browse Assay Genie's current range of RNF40 research reagents below, or read on for the biology behind the target.
RNF40 is an E3 ubiquitin-protein ligase (EC 2.3.2.27) that belongs to the BRE1 family and is found in the nucleus. The protein contains a RING finger, a motif known to mediate protein-protein and protein-DNA interactions. Studies of the rat counterpart suggested that it may function as an E3 ubiquitin-protein ligase and facilitate the ubiquitination and degradation of syntaxin 1, an essential component of the neurotransmitter release machinery.
The RNF40 protein was reported to interact with the tumour suppressor protein RB1. In the context of microbial infection, RNF40 promotes the lytic cycle of human herpesvirus 8 (KSHV) by inducing expression of lytic viral genes, including the latency switch gene RTA/ORF50. The RNF40 gene produces multiple alternatively spliced transcript variants that encode different isoforms.
HGNC classifies the gene, named ring finger protein 40, within the ring finger proteins and the RING finger E3 ubiquitin protein ligases. UniProt lists the protein as E3 ubiquitin-protein ligase BRE1B, and other names in use include KIAA0661, RBP95 and STARING. Mouse and rat both carry an orthologous Rnf40 gene, which allows the function of the RNF40 gene to be examined in rodent systems.
RNF40 is a RING finger E3 ligase, making it relevant to studies of ubiquitin transfer and substrate-specific protein degradation.
RNF40 promotes the KSHV lytic cycle by inducing lytic genes including RTA/ORF50, so it is of interest in herpesvirus latency and reactivation.
Rat studies suggest RNF40 promotes ubiquitination and degradation of syntaxin 1, a component of the neurotransmitter release machinery.
RNF40 was reported to interact with the tumour suppressor RB1, pointing to a possible role in RB1-related regulatory studies.
RNF40 is a nuclear RING finger E3 ubiquitin-protein ligase. Rat studies suggest it facilitates ubiquitination and degradation of syntaxin 1, and it was reported to interact with RB1. It also promotes the KSHV lytic cycle by inducing lytic viral genes such as RTA/ORF50.
RNF40 is also called BRE1B, RBP95, STARING and KIAA0661, and UniProt names it E3 ubiquitin-protein ligase BRE1B. The gene gives rise to multiple alternatively spliced transcript variants that encode different isoforms, so check which isoform a given reagent recognises.
Yes. Mouse and rat both have an orthologous gene named Rnf40, and the NCBI summary draws on studies of the rat counterpart for the syntaxin 1 link. Check the datasheet of any product for its validated reactivity.
Other RING finger E3 ubiquitin protein ligases from the RNF family with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt O75150; product specifications from Assay Genie datasheets.