Anti-RNF40 Antibody (CAB9598)

SKU:
CAB9598
€129 - €349

Description

Technical Manual

Anti-RNF40 Antibody

Product Name:Anti-RNF40 Antibody
Product SKU:CAB9598
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]

Immunogen:A synthesized peptide derived from human RNF40
Sequence:

Email for sequence

Gene ID:9810
Swiss Prot:O75150
Synonyms:BRE1B; RBP95; STARING
Calculated MW:130kDa
Observed MW:130KDa
Reactivity:Human, Mouse
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry
Positive Samples:MCF7, BxPC-3, A375, Mouse brain
Cellular Location:

Product Images

Anti-RNF40 Antibody (CAB9598)

Western blot - RNF40 Rabbit mAb (CAB9598)

Uniprot Information

UniProt Protein Function:RNF40: Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Component of the RNF20/40 complex (also known as BRE1 complex) probably composed of 2 copies of RNF20/BRE1A and 2 copies of RNF40/BRE1B. Interacts with UBE2E1/UBCH6. Interacts with RB1 and WAC. Ubiquitously expressed. Expressed at higher level in testis, heart and pancreas, while it is only weakly expressed in lung, skeletal muscle and small intestine. Belongs to the BRE1 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system; Ligase; Ubiquitin ligase; EC 6.3.2.-; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 16p11.2-p11.1

Cellular Component: nucleoplasm; neuron projection; membrane; nucleus; ubiquitin ligase complex

Molecular Function:protein binding; protein homodimerization activity; mRNA 3'-UTR binding; zinc ion binding; ubiquitin protein ligase binding; protein complex binding; syntaxin-1 binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: histone monoubiquitination; ubiquitin-dependent protein catabolic process; regulation of mitotic cell cycle; histone H2B ubiquitination

NCBI Summary:The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]
UniProt Code:O75150
NCBI GenInfo Identifier:313104295
NCBI Gene ID:9810
NCBI Accession:O75150.4
UniProt Secondary Accession:O75150,Q6AHZ6, Q6N005, Q7L3T6, Q8N615, Q96T18, Q9BSV9 Q9HC82,
UniProt Related Accession:O75150
Molecular Weight:1001
NCBI Full Name:E3 ubiquitin-protein ligase BRE1B
NCBI Synonym Full Names:ring finger protein 40, E3 ubiquitin protein ligase
NCBI Official Symbol:RNF40
NCBI Official Synonym Symbols:BRE1B; RBP95; STARING
NCBI Protein Information:E3 ubiquitin-protein ligase BRE1B; BRE1-B; Rb-associated protein; BRE1 E3 ubiquitin ligase homolog B; 95 kDa retinoblastoma-associated protein; 95 kDa retinoblastoma protein binding protein
UniProt Protein Name:E3 ubiquitin-protein ligase BRE1B
UniProt Synonym Protein Names:95 kDa retinoblastoma-associated protein; RBP95; RING finger protein 40
Protein Family:E3 ubiquitin-protein ligase
UniProt Gene Name:RNF40
UniProt Entry Name:BRE1B_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Signal Transduction
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