IKZF1
IKZF1 (IKAROS family zinc finger 1), also known as Ikaros, hIk-1, LyF-1, PPP1R92 and ZNFN1A1, is a C2H2-type zinc-finger transcription regulator of haematopoietic cell differentiation. It has a central role in the development of lymphocytes, including B and T cells. Browse Assay Genie's current range of IKZF1 research reagents below, or read on for the biology behind the target.
IKZF1 is a transcription regulator that controls haematopoietic cell differentiation and contributes to the development of lymphocytes, B cells and T cells. It binds gamma-satellite DNA, binds and activates the delta-A enhancer element of the CD3-delta gene, and represses the terminal deoxynucleotidyltransferase (TDT) gene during thymocyte differentiation. It regulates transcription through both HDAC-dependent and HDAC-independent complexes. In adult erythrocytes it targets the NuRD and BAF (SWI/SNF) chromatin-remodelling complexes, together as the PYR complex, to the beta-globin locus, and it increases normal apoptosis in adult erythroid cells. It also confers early temporal competence to retinal progenitor cells.
The IKZF1 gene encodes a member of the Ikaros family of zinc-finger DNA-binding proteins associated with chromatin remodelling. Expression of the IKZF1 protein is restricted to the foetal and adult haemo-lymphopoietic system. Alternative splicing gives several isoforms. Most share a C-terminal domain with two zinc fingers that mediate homo- and hetero-dimerisation and interactions with other proteins. The isoforms differ in the number of N-terminal zinc fingers that bind DNA and in the presence of a nuclear localisation signal. Only a few contain the three or more N-terminal fingers needed for high-affinity binding to a core DNA element in target promoters. Isoforms that cannot bind DNA are largely cytoplasmic and are thought to act as dominant-negative factors, so IKZF1 function is isoform-specific.
Overexpression of some dominant-negative isoforms has been associated with B-cell malignancies such as acute lymphoblastic leukaemia. IKZF1 is recognised by CRBN and targeted for degradation by the DCX (DDB1-CUL4-X-box) E3 protein ligase complex. HGNC places the gene in the C2H2-type zinc finger group and among protein phosphatase 1 regulatory subunits, reflected in the alias PPP1R92.
IKZF1 regulates differentiation of haematopoietic cells and contributes to the development of B and T lymphocytes.
IKZF1 acts through HDAC-dependent and HDAC-independent complexes and directs the NuRD and BAF complexes to the beta-globin locus.
Overexpression of dominant-negative IKZF1 isoforms has been associated with B-cell malignancies such as acute lymphoblastic leukaemia.
IKZF1 is recognised by CRBN and targeted for degradation by the DCX E3 ubiquitin ligase complex.
IKZF1 is a transcription regulator of haematopoietic cell differentiation and lymphocyte development. It binds DNA elements such as the CD3-delta enhancer, represses the TDT gene in thymocytes, and works with chromatin-remodelling complexes including NuRD and BAF to regulate target loci.
IKZF1 is also called Ikaros, hIk-1, LyF-1, PPP1R92 and ZNFN1A1, and was formerly named zinc finger protein, subfamily 1A, 1 (Ikaros). Alternative splicing yields isoforms that differ in N-terminal zinc fingers and nuclear localisation signal, so some bind DNA and others do not.
IKZF1 has a mouse ortholog, Ikzf1. Overexpression of some dominant-negative isoforms has been associated with B-cell malignancies such as acute lymphoblastic leukaemia. Check the datasheet of any product for its validated reactivity.
Other Ikaros family zinc-finger transcription factors with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q13422; product specifications from Assay Genie datasheets.