ERCC1
ERCC1 (ERCC excision repair 1, endonuclease non-catalytic subunit) is a DNA repair protein that partners with the XPF endonuclease (ERCC4) to excise damaged DNA. It is also known as RAD10 and belongs to the ERCC1/RAD10/SWI10 family. Browse Assay Genie's current range of ERCC1 research reagents below, or read on for the biology behind the target.
ERCC1 functions in the nucleotide excision repair pathway and is required for the repair of DNA lesions, including those induced by UV light or formed by electrophilic compounds such as cisplatin. The ERCC1 protein forms a heterodimer with the XPF endonuclease, also known as ERCC4, and this heterodimeric endonuclease catalyses the 5' incision made in the process of excising the DNA lesion. ERCC1 is described as the non-catalytic subunit of the pair, in line with its HGNC full name.
The heterodimer is not limited to nucleotide excision repair. The ERCC1-XPF endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks, which makes it relevant to studies of how cells tolerate and resolve several distinct classes of DNA damage. UniProt places the protein in the ERCC1/RAD10/SWI10 family and records its location in the nucleus and cytoplasm. The ERCC1 gene belongs to the HGNC group of ERCC excision repair associated genes, and its mouse ortholog is Ercc1.
Mutations in the ERCC1 gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter its expression may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found. The last exon of the gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. Earlier names for the gene include excision repair cross-complementation group 1 and excision repair cross-complementing rodent repair deficiency, complementation group 1, reflecting its identification through rodent repair-deficient cell lines.
ERCC1 is required for repairing UV-induced and other bulky DNA lesions, as part of the heterodimeric endonuclease that makes the 5' incision.
Because ERCC1 is needed to repair cisplatin-type lesions, it is studied in relation to how cells handle DNA damage from electrophilic compounds.
The ERCC1-XPF endonuclease participates in inter-strand crosslink repair and recombinational DNA repair, extending its relevance beyond excision repair.
Mutations in ERCC1 cause cerebrooculofacioskeletal syndrome, and expression-altering polymorphisms may contribute to carcinogenesis.
ERCC1 acts in nucleotide excision repair, which removes DNA lesions caused by UV light or by electrophilic compounds such as cisplatin. It forms a heterodimeric endonuclease with XPF (ERCC4) that makes the 5' incision, and the complex also contributes to recombinational repair and inter-strand crosslink repair.
ERCC1 is also called RAD10. Older names are excision repair cross-complementation group 1 and excision repair cross-complementing rodent repair deficiency, complementation group 1. Multiple transcript variants encoding different isoforms have been reported, so check the datasheet of any product for its validated reactivity and isoform coverage.
Mutations in the ERCC1 gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter its expression may play a role in carcinogenesis. A mouse ortholog, Ercc1, exists. The protein belongs to the ERCC1/RAD10/SWI10 family, which includes the RAD10 protein name used for this gene.
Other ERCC excision repair genes, including the XPF partner ERCC4, and related DNA repair targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P07992; product specifications from Assay Genie datasheets.