NT5C1A
NT5C1A (5'-nucleotidase, cytosolic IA, also known as CN-I, CN-IA or CN1A) is a cytosolic enzyme that dephosphorylates nucleoside monophosphates. AMP is its major substrate, and it belongs to the 5'-nucleotidase type 3 family. Browse Assay Genie's current range of NT5C1A research reagents below, or read on for the biology behind the target.
NT5C1A catalyses the hydrolysis of ribonucleotide and deoxyribonucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside. AMP is the major substrate, but the enzyme can also hydrolyse dCMP and IMP. As a cytosolic nucleotidase, it acts on nucleoside monophosphates, a reaction first described for this group of enzymes by Hunsucker and colleagues in 2001.
The human NT5C1A gene encodes cytosolic 5'-nucleotidase 1A, a protein that localises to the cytoplasm. UniProt places it in the 5'-nucleotidase type 3 family, and HGNC classifies the gene within the 5'-nucleotidases group. Three EC numbers are assigned to the enzyme (3.1.3.5, 3.1.3.89 and 3.1.3.99), reflecting its activity towards several nucleotide monophosphate substrates. The mouse ortholog is Nt5c1a.
The protein has appeared in the literature under several names, including CN-I, CN-IA, CN1A and CN1, as well as the legacy clone identifiers MGC119199 and MGC119201. These aliases are worth searching when reviewing older publications on cytosolic nucleotidases. Researchers studying nucleotide turnover, AMP dephosphorylation or the wider 5'-nucleotidase group will find NT5C1A a useful reference point when choosing an NT5C1A antibody, NT5C1A ELISA kit or recombinant NT5C1A protein for their work.
NT5C1A hydrolyses AMP, dCMP and IMP to nucleosides and inorganic phosphate, making it relevant to studies of nucleoside monophosphate dephosphorylation.
With AMP as its major substrate, NT5C1A is a model cytosolic 5'-nucleotidase for characterising phosphate release and substrate preference.
As a member of the 5'-nucleotidase type 3 family, NT5C1A supports comparative work across the cytosolic nucleotidase group.
NT5C1A is a cytoplasmic enzyme, so it is a suitable subject for work on cytosolic nucleotide-processing pathways.
NT5C1A catalyses the hydrolysis of ribonucleotide and deoxyribonucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside. AMP is the major substrate, though the enzyme can also hydrolyse dCMP and IMP. It acts in the cytoplasm as a cytosolic nucleotidase.
NT5C1A is the HGNC symbol for 5'-nucleotidase, cytosolic IA. UniProt calls the protein cytosolic 5'-nucleotidase 1A. Aliases and previous symbols include CN-I, CN-IA, CN1A, CN1, MGC119199 and MGC119201, which may appear in older papers and databases.
Yes, the mouse ortholog is Nt5c1a. The human protein belongs to the 5'-nucleotidase type 3 family. For work in other species, check the datasheet of any product for its validated reactivity.
Other targets with similar gene symbols at Assay Genie; use these for browsing related research areas.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9BXI3; product specifications from Assay Genie datasheets.