ALDOB
ALDOB (aldolase, fructose-bisphosphate B) is a tetrameric class I aldolase that cleaves fructose 1,6-bisphosphate in glycolysis and drives the reverse reaction in gluconeogenesis. It also metabolises fructose 1-phosphate in fructolysis and has a non-enzymatic tumour suppressor role. Browse Assay Genie's current range of ALDOB research reagents below, or read on for the biology behind the target.
ALDOB catalyses the aldol cleavage of fructose 1,6-bisphosphate into the two triosephosphates dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate in glycolysis. It also performs the reverse stereospecific aldol addition in gluconeogenesis. In fructolysis, ALDOB metabolises fructose 1-phosphate, which is produced when fructokinase phosphorylates dietary fructose, into dihydroxyacetone phosphate and D-glyceraldehyde.
Independently of its enzymatic activity, ALDOB acts as an adapter and has a tumour suppressor role. It stabilises a ternary complex with G6PD and TP53, which inhibits G6PD activity and keeps oxidative pentose phosphate metabolism in check. The protein is found in the cytosol and at centriolar satellites of the centrosome.
The ALDOB gene encodes one of three vertebrate aldolase isozymes, A, B and C, which differ in their electrophoretic and catalytic properties. They are products of related housekeeping genes with developmentally regulated expression. The embryo produces aldolase A, which is also abundant in adult muscle. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. ALDOB belongs to the class I fructose-bisphosphate aldolase family, and defects in the gene cause hereditary fructose intolerance. Researchers studying the ALDOB protein or running an ALDOB antibody or ALDOB ELISA kit workflow will find the mouse and rat orthologs, both named Aldob, useful for model work.
ALDOB converts fructose 1-phosphate from fructokinase activity into triosephosphate intermediates, making it central to studies of dietary fructose handling.
Defects in ALDOB cause hereditary fructose intolerance, so the gene and protein are a focus for studies of this metabolic disorder.
The enzyme cleaves and forms fructose 1,6-bisphosphate, linking it to the reversible control of carbohydrate flux in glycolysis and gluconeogenesis.
ALDOB stabilises a complex with G6PD and TP53 to restrain G6PD and oxidative pentose phosphate metabolism, a non-enzymatic role relevant to cancer biology.
ALDOB catalyses the cleavage of fructose 1,6-bisphosphate to dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate in glycolysis, and the reverse reaction in gluconeogenesis. It also metabolises fructose 1-phosphate in fructolysis. Separately, it acts as an adapter with G6PD and TP53 to restrain pentose phosphate metabolism.
ALDOB is also known as aldolase B or fructose-bisphosphate aldolase B, with the full name aldolase, fructose-bisphosphate B. Vertebrates have three isozymes, A, B and C. Aldolase B is the form produced in adult liver, kidney and intestine, where aldolase A expression is repressed.
The gene has orthologs in mouse and rat, both called Aldob. Defects in human ALDOB cause hereditary fructose intolerance. The aldolases are related housekeeping genes, and ALDOB belongs to the class I fructose-bisphosphate aldolase family. Check the datasheet of any product for its validated reactivity.
Other aldolase isozymes, fructose and glucose metabolism enzymes and tumour suppressor partners with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P05062; product specifications from Assay Genie datasheets.