PRKD1
PRKD1 (protein kinase D1) is a serine/threonine kinase, also known as PKD1, PKCM, PKC-mu and PRKCM, that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC. It acts in Golgi trafficking, cell survival, migration and differentiation. Browse Assay Genie's current range of PRKD1 research reagents below, or read on for the biology behind the target.
PRKD1 is a serine/threonine-protein kinase that turns short-lived DAG signals into sustained cellular responses downstream of PKC. It is involved in MAPK8/JNK1 and Ras signalling, MAPK1/3 (ERK1/2) signalling, NF-kappa-B-dependent cell survival, cell migration and differentiation. For example, it phosphorylates the epidermal growth factor receptor (EGFR), which suppresses EGF-induced JNK1 activation, and it phosphorylates RIN1, increasing RIN1 competition with RAF1 for binding to GTP-bound Ras.
At the trans-Golgi network, PRKD1 acts downstream of the heterotrimeric G protein beta/gamma complex to maintain Golgi membrane integrity and regulate the fission of transport vesicles bound for the plasma membrane. It may do so by activating phosphatidylinositol 4-kinase beta (PI4KB). In neurons it supports polarity and dendritic arborisation. Elsewhere it phosphorylates HDAC5 and HDAC7, driving their nuclear export in cardiac hypertrophy, VEGFA-induced angiogenesis and BMP2-induced osteoblast differentiation. It also phosphorylates TLR5, NLRP3, TRPV1 and OXTR, linking it to inflammatory responses, inflammasome assembly, pain transmission and social behaviour.
The PRKD1 gene belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, PKD subfamily, and is classed by HGNC in the pleckstrin homology domain containing and protein kinase D groups. The protein was formerly named protein kinase C, mu, which explains the aliases PKC-mu and PRKCM. During apoptosis triggered by cytarabine and other genotoxic agents, caspase-3 cleaves PRKD1, activating its kinase function. Researchers studying PRKD1 often use an antibody, ELISA kit or recombinant protein to follow its expression and signalling; mouse and rat both carry a Prkd1 ortholog.
PRKD1 maintains Golgi membrane integrity and regulates fission of transport vesicles at the trans-Golgi network, making it central to secretory pathway studies.
Downstream of novel PKC, PRKD1 phosphorylates HDAC5 and cardiac troponin I, shaping myocyte hypertrophy and pathological cardiac remodelling.
The PRKD1-HDAC5 pathway mediates VEGFA-induced gene expression, cell migration and tube formation, and PRKD1 drives HDAC7-dependent endothelial proliferation.
PRKD1 phosphorylates TLR5 and NLRP3, contributing to flagellin-stimulated cytokine production and inflammasome assembly.
PRKD1 is a DAG-responsive serine/threonine kinase acting downstream of PKC. It regulates Golgi membrane integrity and vesicle trafficking, MAPK and Ras signalling, NF-kappa-B-mediated survival, cell migration and differentiation, and has roles in cardiac hypertrophy, angiogenesis and inflammatory responses.
PRKD1 is also called PKD1, PKCM, PKD, PKC-mu and PRKCM. The previous HGNC name was protein kinase C, mu, and the current name is protein kinase D1. UniProt lists it as serine/threonine-protein kinase D1, and its EC number is 2.7.11.13.
Mouse and rat each have a Prkd1 ortholog. The kinase is implicated in cardiac hypertrophy, angiogenesis, genotoxic-induced apoptosis and inflammation, and it helps mediate activated KRAS-driven stabilisation of ZNF304 in colorectal cancer cells. Check the datasheet of any product for its validated reactivity.
Other protein kinases, PKD family members and PRKD1 pathway partners with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q15139; product specifications from Assay Genie datasheets.