FUBP1
FUBP1 (far upstream element binding protein 1, also known as FBP or FUBP) is a single-stranded DNA-binding protein that regulates MYC expression through the far upstream element (FUSE). It can act as both an activator and a repressor of transcription. Browse Assay Genie's current range of FUBP1 research reagents below, or read on for the biology behind the target.
FUBP1 regulates MYC expression by binding to the single-stranded far upstream element (FUSE) located upstream of the MYC promoter. Binding occurs on the non-coding strand and is important for the regulation of c-myc in undifferentiated cells. UniProt annotates the protein as able to act both as an activator and as a repressor of transcription, so its effect on target genes depends on context.
The protein is built from three domains: an amphipathic helix N-terminal domain, a DNA-binding central domain, and a C-terminal transactivation domain containing three tyrosine-rich motifs. The N-terminal domain is thought to repress the activity of the C-terminal domain. Beyond FUSE, FUBP1 binds multiple DNA elements. It is also thought to bind RNA and has 3'-5' helicase activity in vitro on both DNA-DNA and RNA-RNA duplexes. The protein localises to the nucleus, and alternative splicing of the FUBP1 gene produces multiple transcript variants. Mouse and rat orthologs are both named Fubp1.
The protein was originally described as far upstream element (FUSE) binding protein 1, and FBP and FUBP remain in use as aliases. Aberrant expression of the FUBP1 gene has been found in malignant tissues, and the gene is important to neural system and lung development. Binding of the protein to viral RNA is thought to play a role in several viral diseases, including hepatitis C and hand, foot and mouth disease.
FUBP1 binds the far upstream element of MYC and regulates its expression, making it a focus for studies of c-myc regulation in undifferentiated cells.
Aberrant FUBP1 expression has been found in malignant tissues, which makes it a protein of interest for studies of tumour biology.
The FUBP1 gene is important to neural system and lung development, supporting its use in developmental biology studies.
FUBP1 binding to viral RNA is thought to contribute to diseases such as hepatitis C and hand, foot and mouth disease.
FUBP1 is a nuclear single-stranded DNA-binding protein that regulates MYC expression by binding the far upstream element (FUSE) upstream of the MYC promoter. UniProt notes it may act as both an activator and a repressor of transcription. It is also thought to bind RNA.
The HGNC name is far upstream element binding protein 1, previously far upstream element (FUSE) binding protein 1. Aliases and previous symbols include FBP and FUBP. Alternative splicing of the gene results in multiple transcript variants, so a single gene yields several related transcripts.
Aberrant FUBP1 expression has been found in malignant tissues, and binding to viral RNA is thought to play a role in hepatitis C and hand, foot and mouth disease. Mouse and rat both carry a Fubp1 ortholog; check the datasheet of any product for its validated reactivity.
Other transcription regulators, single-stranded nucleic acid-binding proteins and MYC pathway targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q96AE4; product specifications from Assay Genie datasheets.