TM4SF20
TM4SF20 (transmembrane 4 L six family member 20, also known as FLJ22800 and TCCE518) is a polytopic membrane protein of the L6 tetraspanin family. It inhibits regulated intramembrane proteolysis of CREB3L1, and ceramide alters its membrane topology. Browse Assay Genie's current range of TM4SF20 research reagents below, or read on for the biology behind the target.
TM4SF20 is a polytopic transmembrane protein that inhibits regulated intramembrane proteolysis (RIP) of CREB3L1. By restraining this cleavage, it limits CREB3L1 activation and the induction of collagen synthesis. The protein responds to ceramide, which alters TM4SF20 membrane topology; in the presence of ceramide, TM4SF20 instead stimulates RIP activation of CREB3L1.
Ceramide acts during translation by reversing the direction in which transmembrane helices of TM4SF20 are translocated into the endoplasmic reticulum membrane. This mechanism is called regulated alternative translocation (RAT), and it regulates the function of the transmembrane protein. The protein is found in cellular membranes, including the endoplasmic reticulum membrane.
The TM4SF20 gene encodes a member of the four-transmembrane L6 superfamily, classed by UniProt in the L6 tetraspanin family and by HGNC in the transmembrane 4 L six family. Members of this family function in cell proliferation, motility and adhesion through their interactions with integrins. In human brain tissue, the gene is expressed at high levels in the parietal lobe, occipital lobe, hippocampus, pons, white matter, corpus callosum and cerebellum.
Knockout of the homologous gene in mouse (Tm4sf20) results in a neurobehavioural phenotype with suggested enhanced motor coordination. A deletion mutation in the human gene is associated with specific language impairment-5, which makes TM4SF20 of interest in neurodevelopmental research.
TM4SF20 inhibits RIP of CREB3L1, making it a useful subject for studying how membrane proteins control transcription factor activation.
Ceramide reverses transmembrane helix translocation in TM4SF20, providing a model of regulated alternative translocation in the endoplasmic reticulum.
Through its effect on CREB3L1 activation, TM4SF20 influences the induction of collagen synthesis, relevant to extracellular matrix studies.
A deletion in the human gene is linked to specific language impairment-5, and mouse knockout shows a neurobehavioural phenotype.
TM4SF20 is a polytopic transmembrane protein that inhibits regulated intramembrane proteolysis of CREB3L1, limiting its activation and the induction of collagen synthesis. In response to ceramide, which alters its membrane topology, it instead stimulates RIP activation of CREB3L1.
The HGNC full name is transmembrane 4 L six family member 20, and UniProt lists it as Transmembrane 4 L6 family member 20. Aliases include FLJ22800 and TCCE518. It belongs to the transmembrane 4 L six family and the L6 tetraspanin family.
A mouse ortholog, Tm4sf20, exists, and its knockout produces a neurobehavioural phenotype with suggested enhanced motor coordination. In humans, a deletion mutation in the gene is associated with specific language impairment-5. Check the datasheet of any product for its validated reactivity.
TM4SF1, a fellow transmembrane 4 L six family member, and other transmembrane proteins with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q53R12; product specifications from Assay Genie datasheets.