DDOST
DDOST is the 48 kDa subunit of the oligosaccharyltransferase (OST) complex, which starts protein N-glycosylation in the endoplasmic reticulum. It is also known as OST, OST48, WBP1 and KIAA0115. Browse Assay Genie's current range of DDOST research reagents below, or read on for the biology behind the target.
DDOST is a subunit of the oligosaccharyl transferase (OST) complex. This complex catalyses the initial transfer of a defined glycan, Glc(3)Man(9)GlcNAc(2) in eukaryotes, from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. This is the first step in protein N-glycosylation. The process occurs cotranslationally, and the complex associates with the Sec61 complex at the channel-forming translocon that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for maximal enzyme activity, and DDOST is required for the assembly of both SST3A- and SS3B-containing OST complexes.
The DDOST gene encodes a protein that belongs to the DDOST 48 kDa subunit family and is anchored in the ER membrane. According to the NCBI Gene summary, the complex transfers high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum, and it co-purifies with ribosomes. HGNC classifies the gene within the oligosaccharyltransferase complex subunits group, and describes the protein as a non-catalytic subunit. Mouse and rat each have a Ddost ortholog.
DDOST is also implicated in the processing of advanced glycation endproducts (AGEs). These form through non-enzymatic reactions between sugars and proteins or lipids, and are associated with ageing and hyperglycaemia. Earlier names for the gene and protein include OST, OST48, WBP1 and KIAA0115, and the former gene name was dolichyl-diphosphooligosaccharide-protein glycosyltransferase. Researchers studying DDOST often work on N-glycosylation, ER protein biogenesis and glycation biology.
DDOST is part of the OST complex that carries out the first step of protein N-glycosylation on nascent polypeptides.
The OST complex associates with the Sec61 translocon, linking glycosylation to protein translocation across the endoplasmic reticulum.
DDOST is required for assembly of both SST3A- and SS3B-containing OST complexes, making it relevant to complex composition studies.
DDOST is implicated in processing advanced glycation endproducts, which are associated with ageing and hyperglycaemia.
DDOST is a subunit of the oligosaccharyl transferase complex, which transfers a Glc(3)Man(9)GlcNAc(2) glycan from dolichol-pyrophosphate to asparagine in Asn-X-Ser/Thr motifs of nascent polypeptides. This is the first step in N-glycosylation. DDOST is also required for assembly of SST3A- and SS3B-containing OST complexes.
DDOST is the HGNC symbol for dolichyl-diphosphooligosaccharide--protein glycosyltransferase non-catalytic subunit. Aliases and previous symbols include OST, OST48, WBP1 and KIAA0115. UniProt lists the protein as the 48 kDa subunit of the glycosyltransferase, which explains the OST48 name.
Mouse and rat both have a Ddost ortholog. The NCBI Gene summary implicates the DDOST product in processing advanced glycation endproducts, which are associated with ageing and hyperglycaemia. Check the datasheet of any product for its validated reactivity.
Other oligosaccharyltransferase complex subunits, translocon components and N-glycosylation pathway targets.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P39656; product specifications from Assay Genie datasheets.