PSMB6
PSMB6 (proteasome 20S subunit beta 6, also known as Y or DELTA) is a beta subunit of the 20S core proteasome, the multicatalytic complex that degrades most intracellular proteins. Within the core it provides peptidylglutamyl-hydrolyzing, or caspase-like, activity. Browse Assay Genie's current range of PSMB6 research reagents below, or read on for the biology behind the target.
PSMB6 is a component of the 20S core proteasome complex, which is involved in the proteolytic degradation of most intracellular proteins. The 20S core associates with different regulatory particles to perform distinct roles. With two 19S regulatory particles it forms the 26S proteasome, which carries out ATP-dependent degradation of ubiquitinated proteins and helps maintain protein homeostasis by removing misfolded or damaged proteins and proteins whose functions are no longer required.
When the 20S proteasome associates with PA200 or PA28 instead, it mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways, including spermatogenesis (20S-PA200 complex) and the generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Within the 20S core, PSMB6 displays peptidylglutamyl-hydrolyzing activity, also termed postacidic or caspase-like activity, so peptide bond hydrolysis occurs directly after acidic residues.
The 20S core is a highly ordered, ring-shaped structure made of four rings of 28 non-identical subunits: two rings of seven alpha subunits and two rings of seven beta subunits. The PSMB6 gene encodes a member of the proteasome B-type (T1B) family, and alternatively spliced transcript variants encoding multiple isoforms have been observed. Proteasomes are found at high concentration throughout eukaryotic cells and cleave peptides in a non-lysosomal pathway. The protein is annotated in the cytoplasm and nucleus. Previous names include proteasome (prosome, macropain) subunit, beta type, 6.
PSMB6 sits in the 26S proteasome, which removes misfolded or damaged proteins and those no longer required, making it relevant to proteostasis studies.
As a 20S core beta subunit, PSMB6 supports research into ATP-dependent degradation of ubiquitinated proteins by the 26S proteasome.
The 20S-PA28 complex generates a subset of MHC class I-presented antigenic peptides, linking PSMB6-containing proteasomes to antigen presentation research.
Ubiquitin-independent degradation by the 20S-PA200 complex is required in spermatogenesis, a context in which PSMB6-containing proteasomes are studied.
PSMB6 is a beta subunit of the 20S core proteasome. It contributes peptidylglutamyl-hydrolyzing (caspase-like) activity, cleaving peptide bonds after acidic residues, as part of the degradation of most intracellular proteins by the 26S or regulator-bound 20S proteasome.
PSMB6 is also known as Y and DELTA, and the protein is called proteasome subunit beta type-6. Earlier names include proteasome (prosome, macropain) subunit, beta type, 6. Alternatively spliced transcript variants encoding multiple isoforms have been observed for the gene.
Orthologues exist in mouse and rat, both named Psmb6. The protein belongs to the peptidase T1B family, and proteasomes are found throughout eukaryotic cells. Check the datasheet of any product for its validated reactivity.
Other proteasome beta subunits from the same T1B peptidase family, including the immunoproteasome components.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P28072; product specifications from Assay Genie datasheets.