Anti-PSMB6 Antibody (CAB4053)

Antibody Name:	Anti-PSMB6 Antibody
Antibody SKU:	CAB4053
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 35-239 of human PSMB6 (NP_002789.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29, HeLa, HepG2, MCF7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 35-239 of human PSMB6 (NP_002789.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TTIM AVQF DGGV VLGA DSRT TTGS YIAN RVTD KLTP IHDR IFCC RSGS AADT QAVA DAVT YQLG FHSI ELNE PPLV HTAA SLFK EMCY RYRE DLMA GIII AGWD PQEG GQVY SVPM GGMM VRQS FAIG GSGS SYIY GYVD ATYR EGMT KEEC LQFT ANAL ALAM ERDG SSGG VIRL AAIA ESGV ERQV LLGD QIPK FAVA TLPP A
Gene ID:	5694
Uniprot:	P28072
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	25kDa
Observed MW:	23kDa

Synonyms:

PSMB6, DELTA, LMPY, Y

Background:

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The encoded protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

UniProt Protein Function:	PSMB6: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the peptidyl glutamyl-like activity. May catalyze basal processing of intracellular antigens. Belongs to the peptidase T1B family.
UniProt Protein Details:	Protein type:Protease; Proteasome complex; EC 3.4.25.1 Chromosomal Location of Human Ortholog: 17p13 Cellular Component: nucleoplasm; proteasome complex; Golgi apparatus; proteasome core complex; cytoplasm; nucleus; cytosol Molecular Function:threonine endopeptidase activity; endopeptidase activity Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle
NCBI Summary:	The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The encoded protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
UniProt Code:	P28072
NCBI GenInfo Identifier:	20532407
NCBI Gene ID:	5694
NCBI Accession:	P28072.4
UniProt Related Accession:	P28072
Molecular Weight:
NCBI Full Name:	Proteasome subunit beta type-6
NCBI Synonym Full Names:	proteasome subunit beta 6
NCBI Official Symbol:	PSMB6
NCBI Official Synonym Symbols:	Y; LMPY; DELTA
NCBI Protein Information:	proteasome subunit beta type-6
UniProt Protein Name:	Proteasome subunit beta type-6
UniProt Synonym Protein Names:	Macropain delta chain; Multicatalytic endopeptidase complex delta chain; Proteasome delta chain; Proteasome subunit Y
Protein Family:	Proteasome
UniProt Gene Name:	PSMB6
UniProt Entry Name:	PSB6_HUMAN

	Western blot analysis of extracts of various cell lines, using PSMB6 antibody (CAB4053) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
	Immunofluorescence analysis of HeLa cells using PSMB6 Polyclonal Antibody (CAB4053) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

PSMB6 Antibody (PACO11536)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)