Anti-KAT2B Antibody (CAB0066)

Antibody Name:	Anti-KAT2B Antibody
Antibody SKU:	CAB0066
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 583-832 of human KAT2B (NP_003875.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse heart, Mouse brain, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 583-832 of human KAT2B (NP_003875.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KGYG THLM NHLK EYHI KHDI LNFL TYAD EYAI GYFK KQGF SKEI KIPK TKYV GYIK DYEG ATLM GCEL NPRI PYTE FSVI IKKQ KEII KKLI ERKQ AQIR KVYP GLSC FKDG VRQI PIES IPGI RETG WKPS GKEK SKEP RDPD QLYS TLKS ILQQ VKSH QSAW PFME PVKR TEAP GYYE VIRF PMDL KTMS ERLK NRYY VSKK LFMA DLQR VFTN CKEY NPPE SEYY KCAN ILEK FFFS KIKE AGLI DK
Gene ID:	8850
Uniprot:	Q92831
Cellular Location:	Nucleus
Calculated MW:	93kDa
Observed MW:	93kDa

Synonyms:

KAT2B, CAF, P/CAF, PCAF

Background:

CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.

UniProt Protein Function:	PCAF: Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Interacts with SIRT1. Interacts (unsumoylated form) with NR2C1; the interaction promotes transactivation activity. Interacts with EP300, CREBBP and DDX17. Interacts with NCOA1 and NCOA3. Component of a large chromatin remodeling complex, at least composed of MYSM1, KAT2B/PCAF, RBM10 and KIF11/TRIP5. Interacts with NR2C2 (hypophosphorylated and unsumoylated form); the interaction promotes the transactivation activity of NR2C2. Binds to HTLV-1 Tax. Interacts with and acetylates HIV-1 Tat. Interacts with KLF1; the interaction does not acetylate KLF1 and there is no enhancement of its transactivational activity. Interacts with NFE4. Interacts with MECOM. Interacts with E2F1; the interaction acetylates E2F1 augmenting its DNA-binding and transcriptional activity. Ubiquitously expressed but most abundant in heart and skeletal muscle. Belongs to the GCN5 family.
UniProt Protein Details:	Protein type:Nuclear receptor co-regulator; EC 2.3.1.48; Acetyltransferase Chromosomal Location of Human Ortholog: 3p24 Cellular Component: nucleoplasm; nucleus Molecular Function:acetyltransferase activity; chromatin binding; cyclin-dependent protein kinase inhibitor activity; histone acetyltransferase activity; histone deacetylase binding; lysine N-acetyltransferase activity; protein binding; protein complex binding; protein kinase binding; transcription coactivator activity; transcription cofactor activity; transcription factor binding Biological Process: cell cycle arrest; cellular response to insulin stimulus; chromatin remodeling; internal peptidyl-lysine acetylation; N-terminal peptidyl-lysine acetylation; negative regulation of cell proliferation; Notch signaling pathway; peptidyl-lysine acetylation; positive regulation of gene expression, epigenetic; positive regulation of transcription from RNA polymerase II promoter; protein amino acid acetylation; transcription initiation from RNA polymerase II promoter
NCBI Summary:	CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation. [provided by RefSeq, Jul 2008]
UniProt Code:	Q92831
NCBI GenInfo Identifier:	83287776
NCBI Gene ID:	8850
NCBI Accession:	Q92831.3
UniProt Secondary Accession:	Q92831,Q6NSK1,
UniProt Related Accession:	Q92831
Molecular Weight:	93,013 Da
NCBI Full Name:	Histone acetyltransferase KAT2B
NCBI Synonym Full Names:	lysine acetyltransferase 2B
NCBI Official Symbol:	KAT2B
NCBI Official Synonym Symbols:	CAF; PCAF; P/CAF
NCBI Protein Information:	histone acetyltransferase KAT2B
UniProt Protein Name:	Histone acetyltransferase KAT2B
UniProt Synonym Protein Names:	Histone acetyltransferase PCAF; Histone acetylase PCAF; Lysine acetyltransferase 2B; P300/CBP-associated factor; P/CAF
Protein Family:	Histone acetyltransferase
UniProt Gene Name:	KAT2B
UniProt Entry Name:	KAT2B_HUMAN

	Western blot analysis of extracts of various cell lines, using KAT2B antibody (CAB0066) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using KAT2B Rabbit pAb (CAB0066) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
KAT2B Antibody (PACO01306)	p300/CBP Colorimetric Cell-Based ELISA Kit
KAT2B Antibody (PACO02110)
Acetyl-KAT2B (K428) Antibody (PACO06077)
KAT2B Antibody (PACO10039)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)