The KAT2B Antibody (CAB0066) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is highly specific and reactive with human samples, making it ideal for use in Western blot applications. By binding to the KAT2B protein, researchers can detect and analyze its expression in various cell types, providing insights into its function in processes such as chromatin remodeling and cellular differentiation.KAT2B, also known as GCN5, is a histone acetyltransferase that plays a crucial role in regulating gene expression through histone acetylation.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
KAT2B Antibody
SKU:
CAB0066
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CAF, PCAF, P/CAF, KAT2B/PCAF
Positive Sample:
Mouse Heart, Rat Heart
Cellular Localization:
Nucleus.
Calculated MW:
93kDa
Observed MW:
93kDa
CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.
Purification Method
Affinity purification
Gene ID
8850
RRID
AB_2756928
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using KAT2B/PCAF Rabbit pAb (CAB0066) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using KAT2B/PCAF Rabbit pAb (CAB0066) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
