TRA2B
TRA2B (transformer 2 beta homolog, also known as Htra2-beta, PPP1R156 and SFRS10) is a nuclear, sequence-specific RNA-binding protein of the serine/arginine splicing factor family. It helps control pre-mRNA splicing and can either activate or suppress exon inclusion. Browse Assay Genie's current range of TRA2B research reagents below, or read on for the biology behind the target.
TRA2B is a sequence-specific RNA-binding protein that participates in the control of pre-mRNA splicing. Depending on context it can either activate or suppress exon inclusion. It binds to pre-mRNA, and in the case of the survival motor neuron gene it binds the AG-rich SE2 domain in the SMN exon 7 RNA. Acting additively with RBMX, it promotes inclusion of exon 7 of SMN2. It also activates the splicing of MAPT/Tau exon 10.
TRA2B can alter pre-mRNA splicing patterns by antagonising the effects of other splicing regulators, such as RBMX. This places it within a network of competing and cooperating factors that determine which exons are retained in a mature transcript. The protein belongs to the splicing factor SR family and is found in the nucleus. The TRA2B gene encodes a protein that, according to NCBI Gene, plays a role in mRNA processing, splicing patterns and gene expression, and alternative splicing of the gene itself gives rise to multiple transcript variants. Mouse and rat both have a Tra2b ortholog.
The gene has carried several names. Earlier HGNC names include splicing factor, arginine/serine-rich 10 (transformer 2 homolog, Drosophila), reflecting its relationship to the Drosophila transformer-2 gene, and the symbols SFRS10 and PPP1R156 are previous or alias symbols. HGNC places it in the groups of protein phosphatase 1 regulatory subunits and RNA binding motif containing genes. TRA2A is a related target for comparison.
TRA2B can either activate or suppress exon inclusion, making it a useful model for how SR proteins shape splicing patterns and gene expression.
TRA2B binds the AG-rich SE2 domain of SMN exon 7 RNA and acts additively with RBMX to promote exon 7 inclusion.
TRA2B activates splicing of MAPT/Tau exon 10, linking this splicing factor to the regulation of Tau transcript isoforms.
TRA2B alters splicing by antagonising regulators such as RBMX, so it is studied in the balance between competing RNA-binding proteins.
TRA2B is a sequence-specific RNA-binding protein that participates in the control of pre-mRNA splicing. It can activate or suppress exon inclusion, promotes SMN2 exon 7 inclusion together with RBMX, and activates splicing of MAPT/Tau exon 10. It is located in the nucleus.
TRA2B is also called Htra2-beta, PPP1R156 and SFRS10. Previous names include splicing factor, arginine/serine-rich 10 (transformer 2 homolog, Drosophila). UniProt lists the protein as Transformer-2 protein homolog beta. Alternative splicing of the gene produces multiple transcript variants.
Yes. Mouse and rat each have an ortholog named Tra2b. The human protein belongs to the splicing factor SR family, and its name reflects a relationship to Drosophila transformer-2. Check the datasheet of any product for its validated reactivity.
TRA2A, the closest named relative of TRA2B, along with other targets whose symbols begin with TRA.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P62995; product specifications from Assay Genie datasheets.