NTMT1
NTMT1 (N-terminal Xaa-Pro-Lys N-methyltransferase 1, also known as NRMT1, METTL11A and AD-003) is a nuclear alpha-N-methyltransferase. It methylates the exposed N-terminus of proteins carrying an [Ala/Gly/Pro/Ser]-Pro-Lys motif, including RCC1, SET and RB. Browse Assay Genie's current range of NTMT1 research reagents below, or read on for the biology behind the target.
NTMT1 is a distributive alpha-N-methyltransferase. It acts on proteins whose initiator methionine has been cleaved, leaving an N-terminal [Ala/Gly/Pro/Ser]-Pro-Lys motif. The enzyme catalyses mono-, di- or tri-methylation of the exposed alpha-amino group of Ala, Gly or Ser in the [Ala/Gly/Ser]-Pro-Lys motif, and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Some substrates may be primed by NTMT2-mediated monomethylation. NTMT1 also catalyses trimethylation of the N-terminal Gly of CENPA after removal of Met-1.
Reported substrates include KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Through its action on RCC1, the regulator of chromosome condensation 1 and guanine nucleotide exchange factor for RAN, NTMT1 is required during mitosis for normal bipolar spindle formation and chromosome segregation. Researchers studying the NTMT1 protein therefore use it as a model for N-terminal methylation in cell division and chromatin biology.
The NTMT1 gene belongs to the methyltransferase superfamily, NTM1 family, and is classed by HGNC among the 7BS protein methyltransferases and the N- and C-terminal methyltransferases. The protein localises to the nucleus. The gene has appeared in the literature under several names, including NRMT1, NRMT, HOMT1A, AD-003, chromosome 9 open reading frame 32 (C9orf32) and methyltransferase like 11A (METTL11A). Mouse and rat both carry an orthologous Ntmt1 gene.
NTMT1 is required for normal bipolar spindle formation and chromosome segregation through its methylation of RCC1.
NTMT1 methylates the alpha-amino group of Ala, Gly, Ser or Pro in defined N-terminal motifs, making it a core enzyme for this modification.
NTMT1 trimethylates the N-terminal Gly of CENPA after Met-1 removal, linking the enzyme to centromeric protein regulation.
Substrates such as SET, RB1, MYL2, MYL3, RPL23A and KLHL31 give NTMT1 relevance across several cellular processes.
NTMT1 is a distributive alpha-N-methyltransferase that mono-, di- or tri-methylates the N-terminal residue of proteins with an [Ala/Gly/Pro/Ser]-Pro-Lys motif once the initiator Met is removed. Substrates include RCC1, SET, RB1 and CENPA, and its action on RCC1 supports mitotic spindle formation.
NTMT1 is also called NRMT1, NRMT, METTL11A, HOMT1A, AD-003 and C9orf32. Previous HGNC names were chromosome 9 open reading frame 32 and methyltransferase like 11A. NTMT2 is a related enzyme whose monomethylation may prime some NTMT1 substrates.
Yes. Mouse and rat each have an orthologous gene named Ntmt1. The human protein belongs to the methyltransferase superfamily, NTM1 family. For experimental work, check the datasheet of any product for its validated reactivity.
Substrates, the related enzyme NTMT2 and mitosis-associated proteins linked to NTMT1 function.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9BV86; product specifications from Assay Genie datasheets.