PARP4
PARP4 (poly(ADP-ribose) polymerase family member 4, also known as VAULT3, p193, VPARP, VWA5C, ARTD4 and ADPRTL1) is a mono-ADP-ribosyltransferase of the ARTD/PARP family. It modifies target proteins by mono-ADP-ribosylation and is a component of the vault particle. Browse Assay Genie's current range of PARP4 research reagents below, or read on for the biology behind the target.
PARP4 is a mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins. It is also a component of the vault particle, a massive ribonucleoprotein complex that may play a role in cargo transport and signal transduction. UniProt lists the protein in the cytoplasm, the nucleus and the spindle of the cytoskeleton.
The PARP4 gene encodes a protein that the NCBI Gene summary describes as capable of catalysing a poly(ADP-ribosyl)ation reaction. Its catalytic domain is homologous to that of poly(ADP-ribose) polymerase 1 (PARP1). However, it lacks the N-terminal DNA-binding domain that activates the C-terminal catalytic domain in that enzyme. Because PARP4 cannot bind DNA directly, its transferase activity may instead be activated by other factors, for example through protein-protein interactions mediated by its extensive carboxyl terminus.
PARP4 belongs to the ARTD/PARP family and sits in the HGNC gene group of poly(ADP-ribose) polymerases. Its earlier name, ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 1, explains the legacy symbol ADPRTL1. The alternative names VAULT3, p193 and VPARP reflect its association with the vault particle. The mouse ortholog is Parp4. Researchers studying PARP4 often work with the PARP4 protein, PARP4 antibody reagents or a PARP4 ELISA kit to examine its expression and function.
PARP4 mediates mono-ADP-ribosylation of target proteins, making it relevant to work on this post-translational modification within the ARTD/PARP family.
As a component of the vault particle, PARP4 is relevant to research on this ribonucleoprotein complex and its possible role in cargo transport and signal transduction.
PARP4 has a catalytic domain homologous to PARP1 but lacks the N-terminal DNA-binding domain, which makes it useful for comparing PARP family regulation.
Its transferase activity may be activated by protein-protein interactions through the extensive carboxyl terminus, which makes PARP4 a subject for studies of non-DNA-dependent activation.
PARP4 is a mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins. It is also a component of the vault particle, a large ribonucleoprotein complex that may play a role in cargo transport and signal transduction. Check the datasheet of any product for its validated reactivity.
PARP4 is also called VAULT3, p193, VPARP, VWA5C, ARTD4 and ADPRTL1. The HGNC previous name is ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 1. UniProt names the protein as protein mono-ADP-ribosyltransferase PARP4.
PARP4 has a catalytic domain homologous to that of PARP1 but lacks the N-terminal DNA-binding domain that activates PARP1. As it cannot bind DNA directly, its activity may be regulated by other factors, such as protein-protein interactions through its long carboxyl terminus. The mouse ortholog is Parp4.
Other members of the PARP family of ADP-ribosyltransferases with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9UKK3; product specifications from Assay Genie datasheets.