CENPE
CENPE (centromere protein E, also known as KIF10 and PPP1R61) is a microtubule plus-end-directed kinesin motor found at the kinetochore. It drives chromosome congression, stabilises kinetochore-microtubule attachment and contributes to spindle assembly checkpoint activation during mitosis. Browse Assay Genie's current range of CENPE research reagents below, or read on for the biology behind the target.
CENPE is a microtubule plus-end-directed kinetochore motor with an important role in chromosome congression, microtubule-kinetochore conjugation and spindle assembly checkpoint (SAC) activation. It drives congression, the alignment of chromosomes at the spindle equator to form the metaphase plate, by mediating the lateral sliding of polar chromosomes along spindle microtubules. It also helps establish and maintain connections between kinetochores and spindle microtubules. Transport of pole-proximal chromosomes towards the equator is favoured by detyrosinated microtubule tracks. CENPE acts as a processive, bi-directional tracker of dynamic microtubule tips, and after congression it continues to strengthen kinetochore links with dynamic microtubule ends.
The CENPE protein suppresses chromosome congression in NDC80-depleted cells and contributes positively only when microtubules are stabilised. Stable kinetochore-microtubule attachment also requires CENPE-dependent localisation of other proteins to the kinetochore, including BUB1B, MAD1 and MAD2. Through its interaction with BUB1B, CENPE activates the kinase activity of BUB1B, which is important for SAC activation. It is necessary for the mitotic checkpoint signal at individual kinetochores, preventing aneuploidy caused by single chromosome loss.
The CENPE gene encodes a kinesin-like protein that accumulates in the G2 phase of the cell cycle. It is absent during interphase and first appears at the centromere region of chromosomes during prometaphase. Spindle microtubule capture at kinetochores depends on it, and it couples chromosome position to microtubule depolymerising activity. The protein belongs to the kinesin family within the myosin-kinesin ATPase superfamily. HGNC places it in the kinesin and protein phosphatase 1 regulatory subunit gene groups. Alternative splicing produces multiple transcript variants encoding distinct isoforms. Earlier names refer to its size, as centromere protein E (312kD).
CENPE mediates lateral sliding of polar chromosomes along spindle microtubules to the equator, making it central to metaphase plate formation.
CENPE interacts with BUB1B to activate its kinase activity, supporting SAC signalling at individual kinetochores.
CENPE helps form stable kinetochore attachments to spindle microtubules and localises BUB1B, MAD1 and MAD2 to the kinetochore.
CENPE is necessary for the mitotic checkpoint signal that prevents aneuploidy caused by loss of single chromosomes.
CENPE is a kinetochore-associated kinesin motor that moves along microtubules towards their plus ends. It drives chromosome congression, stabilises kinetochore-microtubule attachments and helps activate the spindle assembly checkpoint through BUB1B, so that chromosomes segregate accurately in mitosis.
CENPE is also called KIF10 and PPP1R61, and its protein is named centromere-associated protein E. Earlier names include centromere protein E (312kD). NCBI notes that alternative splicing yields several transcript variants encoding distinct isoforms.
CENPE has a mouse ortholog, Cenpe. The protein belongs to the kinesin family of the myosin-kinesin ATPase superfamily. For any CENPE antibody or ELISA kit, check the datasheet of any product for its validated reactivity.
Spindle checkpoint proteins, kinetochore components and other kinesins that act alongside CENPE in mitosis.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q02224; product specifications from Assay Genie datasheets.