PEF1
PEF1 (penta-EF-hand domain containing 1), also known as peflin or PEF1A, is a calcium-binding adapter protein that bridges proteins or stabilises weak protein-protein complexes in response to calcium. It partners with PDCD6 to influence endoplasmic reticulum (ER)-Golgi transport. Browse Assay Genie's current range of PEF1 research reagents below, or read on for the biology behind the target.
PEF1 is a calcium-binding protein that acts as an adapter, bridging unrelated proteins or stabilising weak protein-protein complexes when calcium is present. Together with PDCD6, it acts as a calcium-dependent adapter for the BCR(KLHL12) complex, which is involved in ER-Golgi transport through its control of COPII coat size. When cytosolic calcium rises, the PEF1-PDCD6 heterodimer interacts with the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B) and brings them together. This promotes monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification.
The role of PEF1 within the heterodimer is not settled. Some evidence shows that PEF1 and PDCD6 work together to promote association between PDCD6 and SEC31 in the presence of calcium. Other reports show that PEF1 dissociates from PDCD6 when calcium is present and may act as a negative regulator of PDCD6. PEF1 is also described as a negative regulator of ER-Golgi transport, possibly by inhibiting the interaction between PDCD6 and SEC31. UniProt lists its locations as the cytoplasm, endoplasmic reticulum, membrane and COPII-coated vesicle membrane.
The PEF1 gene encodes a member of the penta-EF-hand protein family and belongs to the HGNC group of EF-hand domain containing genes. NCBI Gene notes that the protein forms a heterodimer with the programmed cell death 6 gene product and may modulate its function in Ca(2+) signalling. Alternative splicing produces multiple transcript variants, and a pseudogene has been identified on chromosome 1. Mouse and rat both carry a Pef1 ortholog.
PEF1 is described as a negative regulator of ER-Golgi transport, making it relevant to studies of how secretory trafficking is controlled.
With PDCD6, PEF1 acts as an adapter for the BCR(KLHL12) complex, which regulates the size of COPII coats.
PEF1 is a penta-EF-hand protein that may modulate PDCD6 function in Ca(2+) signalling, linking calcium changes to protein complex assembly.
PEF1-PDCD6 signalling promotes SEC31 monoubiquitination and collagen export, which is required for neural crest specification.
PEF1 is a calcium-binding adapter protein that bridges proteins or stabilises weak complexes in response to calcium. With PDCD6, it links the BCR(KLHL12) complex to SEC31, promoting SEC31 monoubiquitination and collagen export. It is also described as a negative regulator of ER-Golgi transport.
PEF1 is the HGNC symbol for penta-EF-hand domain containing 1. UniProt calls the protein peflin, and PEF1A is an alias. NCBI Gene notes that alternative splicing gives rise to multiple transcript variants, so several transcripts may be reported for this gene.
Yes. Mouse and rat each have an ortholog named Pef1, so the gene can be studied in common rodent models. As ever, check the datasheet of any product for its validated reactivity.
PEF1 partners, COPII coat components and other calcium-binding proteins involved in ER-Golgi transport.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9UBV8; product specifications from Assay Genie datasheets.