MAGED1
MAGED1 (MAGE family member D1, also known as NRAGE or DLXIN-1) is a member of the melanoma antigen gene family. It takes part in neurotrophin receptor-mediated apoptosis, restrains cell cycle progression and contributes to circadian rhythm regulation. Browse Assay Genie's current range of MAGED1 research reagents below, or read on for the biology behind the target.
MAGED1 is involved in the apoptotic response that follows nerve growth factor (NGF) binding in neuronal cells. It inhibits cell cycle progression and facilitates NGFR-mediated apoptosis, and the gene has been shown to act in the p75 neurotrophin receptor-mediated programmed cell death pathway. It may also regulate the function of DLX family members, which is reflected in the alias DLXIN-1.
The protein is also linked to ubiquitin signalling. It may enhance the ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases, and is proposed to act by recruiting or stabilising the Ubl-conjugating enzyme (E2) at the E3:substrate complex. In circadian biology, MAGED1 plays a role in rhythm regulation and may act as a RORA co-regulator. In this role it would modulate core clock genes, with BMAL1 and NFIL3 induced and NR1D1 repressed.
The MAGED1 gene belongs to the melanoma antigen gene (MAGE) family. Most family members encode tumour-specific antigens that are not expressed in normal adult tissues apart from testis. MAGED1 differs: despite strong homology to other MAGE proteins, it is expressed in almost all normal adult tissues. Three transcript variants encoding two isoforms have been reported. UniProt places the protein in the cytoplasm, cell membrane and nucleus. The previous name was melanoma antigen family D1, and NRAGE is a widely used alias. Mouse and rat both carry a Maged1 ortholog.
MAGED1 is involved in the apoptotic response after NGF binding and facilitates NGFR-mediated cell death in neuronal cells.
The protein inhibits cell cycle progression, making it relevant to studies of growth control and programmed cell death.
MAGED1 may enhance RING-type E3 ligase activity by recruiting or stabilising the E2 enzyme at the E3:substrate complex.
MAGED1 may act as a RORA co-regulator, modulating the expression of clock genes including BMAL1, NFIL3 and NR1D1.
MAGED1 is involved in apoptosis after NGF binding in neuronal cells, inhibits cell cycle progression and facilitates NGFR-mediated apoptosis. It may also enhance RING-type E3 ubiquitin ligase activity, regulate DLX family function and contribute to circadian rhythm regulation as a possible RORA co-regulator.
MAGED1 is also known as NRAGE and DLXIN-1, and its previous name was melanoma antigen family D1. NCBI Gene reports three transcript variants that encode two different isoforms. Check the datasheet of any product for the isoform or region it targets.
No. Most MAGE family genes encode tumour-specific antigens absent from normal adult tissues except testis. MAGED1 shares strong homology with them but is expressed in almost all normal adult tissues. Mouse and rat both have a Maged1 ortholog.
Neurotrophin signalling, circadian clock and other apoptosis-related targets connected to MAGED1 biology.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9Y5V3; product specifications from Assay Genie datasheets.