PNCK
PNCK (pregnancy up-regulated nonubiquitous CaM kinase, also known as CaMK1b) is a calcium/calmodulin-dependent serine/threonine protein kinase of the CAMK1 family. It is proposed to act in a calcium-triggered signalling cascade and can phosphorylate and activate CAMK1. Browse Assay Genie's current range of PNCK research reagents below, or read on for the biology behind the target.
PNCK is a calcium/calmodulin-dependent protein kinase that belongs to a proposed calcium-triggered signalling cascade. As a serine/threonine kinase, it transfers phosphate groups onto substrate proteins in response to calcium signals. In vitro, PNCK phosphorylates the transcription factor CREB1 and the synaptic vesicle-associated protein SYN1 (synapsin I). It also phosphorylates and activates CAMK1, which places it upstream of another member of the same kinase family.
UniProt assigns the protein to the protein kinase superfamily, in the CAMK Ser/Thr protein kinase family and the CaMK subfamily. HGNC lists the PNCK gene in the CAMK1 family, and NCBI Gene describes PNCK as a member of the calcium/calmodulin-dependent protein kinase family of serine/threonine kinases. The enzyme is classified as EC 2.7.11.17. UniProt records its subcellular locations as the cytoplasm and the nucleus. Mouse and rat both carry a Pnck ortholog.
The name reflects the original description of the gene: pregnancy up-regulated non-ubiquitously expressed CaM kinase. The protein is also recorded under the UniProt name calcium/calmodulin-dependent protein kinase type 1B, with the aliases CaMK1b and MGC45419. Researchers studying calcium signalling, kinase cascades and CREB1 or synapsin phosphorylation may use a PNCK antibody, PNCK ELISA kit or recombinant PNCK protein to examine this kinase.
PNCK is proposed to function in a calcium-triggered signalling cascade, making it relevant to studies of how calcium signals are relayed by kinases.
As a CAMK1 family member that phosphorylates and activates CAMK1, PNCK is useful for dissecting relationships within the calcium/calmodulin-dependent kinase family.
In vitro, PNCK phosphorylates CREB1, so it is a candidate kinase for work on calcium-regulated transcription factor modification.
PNCK phosphorylates SYN1 (synapsin I) in vitro, linking it to investigations of kinase regulation of synapsin substrates.
PNCK is a calcium/calmodulin-dependent serine/threonine kinase proposed to act in a calcium-triggered signalling cascade. In vitro it phosphorylates CREB1 and SYN1 (synapsin I), and it phosphorylates and activates CAMK1. UniProt locates it in the cytoplasm and nucleus.
PNCK stands for pregnancy up-regulated nonubiquitous CaM kinase. Aliases include CaMK1b and MGC45419, and UniProt names it calcium/calmodulin-dependent protein kinase type 1B. Earlier HGNC names spelled it pregnancy upregulated non-ubiquitously expressed CaM kinase.
Mouse and rat both have a Pnck ortholog, and the human gene is part of the CAMK1 family. Cross-reactivity differs between reagents, so check the datasheet of any product for its validated reactivity.
Other CaM kinase family members and PNCK substrates, including CAMK1, CREB1 and SYN1, with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q6P2M8; product specifications from Assay Genie datasheets.