Anti-CTBP2 Antibody (CAB2257)

Antibody Name:	Anti-CTBP2 Antibody
Antibody SKU:	CAB2257
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human CTBP2 (NP_001320.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-251MG, HeLa, BxPC-3, Mouse brain, Rat brain

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human CTBP2 (NP_001320.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SEPF SFAQ GPLK DAPN LICT PHTA WYSE QASL EMRE AAAT EIRR AITG RIPE SLRN CVNK EFFV TSAP WSVI DQQA IHPE LNGA TYRY PPGI VGVA PGGL PAAM EGII PGGI PVTH NLPT VAHP SQAP SPNQ PTKH GDNR EHPN EQ
Gene ID:	1488
Uniprot:	P56545
Cellular Location:	Cell junction, Nucleus, synapse
Calculated MW:	48kDa/56kDa/106kDa
Observed MW:	47kDa

Synonyms:

CTBP2

Background:

This gene produces alternative transcripts encoding two distinct proteins. One protein is a transcriptional repressor, while the other isoform is a major component of specialized synapses known as synaptic ribbons. Both proteins contain a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases. A portion of the 3' untranslated region was used to map this gene to chromosome 21q21.3; however, it was noted that similar loci elsewhere in the genome are likely. Blast analysis shows that this gene is present on chromosome 10. Several transcript variants encoding two different isoforms have been found for this gene.

UniProt Protein Function:	CTBP2: Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation. Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nuclear receptor co-regulator; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 10q26.13 Cellular Component: transcriptional repressor complex; synapse; cell junction; nucleus Molecular Function:protein binding; retinoic acid receptor binding; transcription coactivator activity; chromatin binding; transcription corepressor activity; NAD binding; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Biological Process: negative regulation of cell proliferation; transcription, DNA-dependent; white fat cell differentiation; positive regulation of retinoic acid receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; viral genome replication; negative regulation of transcription, DNA-dependent
NCBI Summary:	This gene produces alternative transcripts encoding two distinct proteins. One protein is a transcriptional repressor, while the other isoform is a major component of specialized synapses known as synaptic ribbons. Both proteins contain a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases. A portion of the 3' untranslated region was used to map this gene to chromosome 21q21.3; however, it was noted that similar loci elsewhere in the genome are likely. Blast analysis shows that this gene is present on chromosome 10. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]
UniProt Code:	P56545
NCBI GenInfo Identifier:	3182976
NCBI Gene ID:	1488
NCBI Accession:	P56545.1
UniProt Secondary Accession:	P56545,O43449, Q5SQP7, Q69YI3, Q86SV0, Q9H2T8, A8K2X5 D3DRF5,
UniProt Related Accession:	P56545
Molecular Weight:	106,187 Da
NCBI Full Name:	C-terminal-binding protein 2
NCBI Synonym Full Names:	C-terminal binding protein 2
NCBI Official Symbol:	CTBP2
NCBI Protein Information:	C-terminal-binding protein 2; ribeye
UniProt Protein Name:	C-terminal-binding protein 2
Protein Family:	C-terminal-binding protein
UniProt Gene Name:	CTBP2
UniProt Entry Name:	CTBP2_HUMAN

	Western blot analysis of extracts of various cell lines, using CTBP2 Antibody (CAB2257) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of U2OS cells using CTBP2 Rabbit pAb (CAB2257) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-CTBP2 Antibody (CAB16826)

CTBP2 Antibody (PACO02514)

CTBP2 Antibody (PACO08651)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)