Histone-lysine N-methyltransferase, H3 lysine-79 specific (Histone H3-K79 methyltransferase/DOT1-like protein/Lysine N-methyltransferase 4/DOT1L) is a nucleus protein which belongs to the DOT1 family. In contrast to other lysine histone methyltransferase, DOT1L lacks a SET domain, implying the need of another method for lysine histone methylation. DOT1L is an histone methyltransferase. It methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. DOT1L binds to DNA. DOT1L is responsible for methylation of lysine 79 on histone H3 (H3K79). It regulates telomeric silencing, cell cycle checkpoints, development, and transcription. The DOT1L-containing complex was analyzed by mass spectrometry, and it was discovered that AF9, ENL, and NPM1 were important DOT1L-interacting proteins. DOT1L might control AF9- and ENL-mediated transcription, regulate RNA processing, and function as a histone chaperone in a NPM1-dependent manner. Human DOT1L/KMT4 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human DOT1L/KMT4 Recombinant Protein (RPES1182)
Product Code:
RPES1182
Size:
50 µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
DOT1,KMT4
Accession:
NP_115871.1
Sequence:
Gly 2-Lys 416
Fusion tag:
Endotoxin:
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Protein Construction:
A DNA sequence encoding the human DOT1L (NP_115871.1) N-terminal segment (Gly 2-Lys 416) was expressed and purified, with two additional amino acids (Gly & Pro) at the N-terminus.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
