Anti-PLCB1 Antibody (CAB1971)

Antibody Name:	Anti-PLCB1 Antibody
Antibody SKU:	CAB1971
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 917-1216 of human PLCB1 (NP_056007.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	NCI-H460

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 917-1216 of human PLCB1 (NP_056007.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KSFV KLQK KHYK EMKD LVKR HHKK TTDL IKEH TTKY NEIQ NDYL RRRA ALEK SAKK DSKK KSEP SSPD HGSS TIEQ DLAA LDAE MTQK LIDL KDKQ QQQL LNLR QEQY YSEK YQKR EHIK LLIQ KLTD VAEE CQNN QLKK LKEI CEKE KKEL KKKM DKKR QEKI TEAK SKDK SQME EEKT EMIR SYIQ EVVQ YIKR LEEA QSKR QEKL VEKH KEIR QQIL DEKP KLQV ELEQ EYQD KFKR LPLE ILEF VQEA MKGK ISED SNHG SAPL SLSS DPGK VNHK TPSS EELG GDIP GKEF DTPL
Gene ID:	23236
Uniprot:	Q9NQ66
Cellular Location:	Cytoplasm, Nucleus membrane
Calculated MW:	133kDa/138kDa
Observed MW:	139kDa

Synonyms:

PLCB1, EIEE12, PI-PLC, PLC-154, PLC-I, PLC-beta-1, PLC154, PLCB1A, PLCB1B

Background:

The protein encoded by this gene catalyzes the formation of inositol 1, 4, 5-trisphosphate and diacylglycerol from phosphatidylinositol 4, 5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. This gene is activated by two G-protein alpha subunits, alpha-q and alpha-11. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	PLCB1: an enzyme that catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. Binds to calmodulin. Uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. Activated by two G-protein alpha subunits, alpha-q and alpha-11. Two transcript variant isoforms have been described.
UniProt Protein Details:	Protein type:Phospholipase; EC 3.1.4.11; Carbohydrate Metabolism - inositol phosphate Chromosomal Location of Human Ortholog: 20p12 Cellular Component: nuclear membrane; nuclear chromatin; cytoplasm; nuclear speck; cytosol; nucleus Molecular Function:calmodulin binding; signal transducer activity; phosphatidylinositol-4,5-bisphosphate binding; enzyme binding; protein homodimerization activity; calcium ion binding; phosphoinositide phospholipase C activity; GTPase activator activity; lamin binding Biological Process: fat cell differentiation; positive regulation of developmental growth; inositol phosphate metabolic process; acetylcholine receptor signaling, muscarinic pathway; glutamate signaling pathway; metabolic process; positive regulation of interleukin-12 production; positive regulation of transcription, DNA-dependent; positive regulation of JNK cascade; signal transduction; positive regulation of myoblast differentiation; memory; synaptic transmission; positive regulation of embryonic development; insulin-like growth factor receptor signaling pathway; cerebral cortex development; phosphatidylinositol metabolic process; G2/M transition of mitotic cell cycle; negative regulation of transcription, DNA-dependent; regulation of G-protein coupled receptor protein signaling pathway; lipid catabolic process; positive regulation of GTPase activity Disease: Epileptic Encephalopathy, Early Infantile, 12
NCBI Summary:	The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. This gene is activated by two G-protein alpha subunits, alpha-q and alpha-11. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9NQ66
NCBI GenInfo Identifier:	12643814
NCBI Gene ID:	23236
NCBI Accession:	Q9NQ66.1
UniProt Secondary Accession:	Q9NQ66,O60325, Q17RQ6, Q5TFF7, Q5TGC9, Q8IV93, Q9BQW2 Q9H4H2, Q9H8H5, Q9NQ65, D3DW12, D3DW13,
UniProt Related Accession:	Q9NQ66
Molecular Weight:	1216
NCBI Full Name:	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1
NCBI Synonym Full Names:	phospholipase C, beta 1 (phosphoinositide-specific)
NCBI Official Symbol:	PLCB1
NCBI Official Synonym Symbols:	PLC-I; EIEE12; PI-PLC; PLC154; PLCB1A; PLCB1B; PLC-154
NCBI Protein Information:	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1; PLC-beta-1; phospholipase C-I; phosphoinositidase C; inositoltrisphosphohydrolase; triphosphoinositide phosphodiesterase; monophosphatidylinositol phosphodiesterase; 1-phosphatidyl-D-myo-in
UniProt Protein Name:	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1
UniProt Synonym Protein Names:	PLC-154; Phosphoinositide phospholipase C-beta-1; Phospholipase C-I; PLC-I; Phospholipase C-beta-1; PLC-beta-1
Protein Family:	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
UniProt Gene Name:	PLCB1
UniProt Entry Name:	PLCB1_HUMAN

	Western blot analysis of extracts of NCI-H460 cells, using PLCB1 antibody (CAB1971) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat heart using PLCB1 antibody (CAB1971) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human kidney cancer using PLCB1 antibody (CAB1971) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using PLCB1 antibody (CAB1971) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using PLCB1 antibody (CAB1971). Blue: DAPI for nuclear staining.

ELISA Kits

Human 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-1 (PLCB1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)