The TPPP/p25 Antibody (CAB8574) is a high-quality antibody developed for reliable detection and analysis of target proteins. TPPP, also known as tubulin polymerization promoting protein, is involved in microtubule dynamics and has been implicated in neurodegenerative diseases such as Parkinson's and Alzheimer's.This polyclonal antibody, produced in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. By binding to the TPPP protein, this antibody enables accurate detection and analysis in different cell types, making it an ideal choice for studies in neuroscience and neurology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TPPP/p25 Antibody
SKU:
CAB8574
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
p24, p25, TPPP1, TPPP/p25, p25alpha
Positive Sample:
Jurkat, Mouse brain, Mouse pancreas, Rat brain
Cellular Localization:
Cytoplasm, Nucleus, Cytoskeleton.
Calculated MW:
24kDa
Observed MW:
24kDa
Enables several functions, including GTPase activity; magnesium ion binding activity; and protein homodimerization activity. Involved in several processes, including microtubule cytoskeleton organization; negative regulation of tubulin deacetylation; and positive regulation of protein polymerization. Located in several cellular components, including mitochondrion; mitotic spindle; and perinuclear region of cytoplasm. Colocalizes with microtubule and microtubule bundle.
Purification Method
Affinity purification
Gene ID
11076
RRID
AB_2772694
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TPPP/p25 Rabbit pAb (CAB8574) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
