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[KO Validated] Cleaved PARP1 p25 Rabbit Monoclonal Antibody (CAB19612)

SKU:
CAB19612
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
  • KO Validated Antibodies 2 Anti-Cleaved PARP p25 Antibody KO Validated CAB19612
  • KO Validated Antibodies 2 Anti-Cleaved PARP p25 Antibody KO Validated CAB19612
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] Cleaved PARP1 p25 Rabbit Monoclonal Antibody
SKU:CAB19612
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 150-214 of human Cleaved PARP1 p25 (P09874).
Sequence:QLGM IDRW YHPG CFVK NREE LGFR PEYS ASQL KGFS LLAT EDKE ALKK QLPG VKSE GKRK GDEV D
Tested Applications:WB IP ELISA
Recommended Dilution:WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:PARP; PARS; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1; Poly-PARP; Cleaved PARP1 p25
Positive Sample:Jurkat,Mouse spleen,NIH/3T3
Conjugate:Unconjugated
Cellular Localization:Nucleus, nucleolus.
Calculated MW:113kDa
Observed MW:27kDa

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

Purification Method:Affinity purification
Gene ID:142
Clone Number:ARC0091
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Cleaved PARP1 p25 Rabbit mAb (CAB19612) at 1:1000 dilution.Jurkat cells were treated by Staurosporine(1uM) at room temperature for 3 hours .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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