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[KO Validated] PARP1 Rabbit Monoclonal Antibody (CAB19596)

SKU:
CAB19596
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
  • KO Validated Antibodies 2 Anti-PARP Antibody KO Validated CAB19596
  • KO Validated Antibodies 2 Anti-PARP Antibody KO Validated CAB19596
  • KO Validated Antibodies 2 Anti-PARP Antibody KO Validated CAB19596
  • KO Validated Antibodies 2 Anti-PARP Antibody KO Validated CAB19596
  • KO Validated Antibodies 2 Anti-PARP Antibody KO Validated CAB19596
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] PARP1 Rabbit Monoclonal Antibody
SKU:CAB19596
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 700-800 of human PARP1 (P09874).
Sequence:KLSK RQIQ AAYS ILSE VQQA VSQG SSDS QILD LSNR FYTL IPHD FGMK KPPL LNNA DSVQ AKVE MLDN LLDI EVAY SLLR GGSD DSSK DPID VNYE KLKT D
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Synonyms:PARP; PARS; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1; Poly-PARP; PARP1
Positive Sample:Jurkat treated by Staurosporine,Mouse testis,Rat testis
Conjugate:Unconjugated
Cellular Localization:Nucleus, nucleolus.
Calculated MW:113kDa
Observed MW:89kDa/113kDa

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

Purification Method:Affinity purification
Gene ID:142
Clone Number:ARC0075
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from Jurkat cells, using PARP1 Rabbit mAb (CAB19596) at 1:1000 dilution.Jurkat cells were treated by Staurosporine(1uM) at room temperature for 3 hours .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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