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[KO Validated] PARP1 Rabbit Polyclonal Antibody (CAB0942)

SKU:
CAB0942
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
  • KO Validated Antibodies 1 Anti-PARP1 Antibody CAB0942KO Validated
  • KO Validated Antibodies 1 Anti-PARP1 Antibody CAB0942KO Validated
  • KO Validated Antibodies 1 Anti-PARP1 Antibody CAB0942KO Validated
  • KO Validated Antibodies 1 Anti-PARP1 Antibody CAB0942KO Validated
  • KO Validated Antibodies 1 Anti-PARP1 Antibody CAB0942KO Validated
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] PARP1 Rabbit Polyclonal Antibody
SKU:CAB0942
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 81-390 of human PARP1 (NP_001609.2).
Sequence:DQQK VKKT AEAG GVTG KGQD GIGS KAEK TLGD FAAE YAKS NRST CKGC MEKI EKGQ VRLS KKMV DPEK PQLG MIDR WYHP GCFV KNRE ELGF RPEY SASQ LKGF SLLA TEDK EALK KQLP GVKS EGKR KGDE VDGV DEVA KKKS KKEK DKDS KLEK ALKA QNDL IWNI KDEL KKVC STND LKEL LIFN KQQV PSGE SAIL DRVA DGMV FGAL LPCE ECSG QLVF KSDA YYCT GDVT AWTK CMVK TQTP NRKE WVTP KEFR EISY LKKL KVKK QDRI FPPE TSAS VAAT PPPS TASA PAAV NSSA SADK PL
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:1000 - 1:5000 IF/ICC,1:50 - 1:200
Synonyms:PARP; PARS; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1; Poly-PARP; PARP1
Positive Sample:HeLa,Jurkat,A-549,Mouse testis,Rat testis
Conjugate:Unconjugated
Cellular Localization:Nucleus, nucleolus.
Calculated MW:113kDa
Observed MW:89kDa/113kDa

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

Purification Method:Affinity purification
Gene ID:142
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using PARP1 Rabbit pAb (CAB0942) at 1:3000 dilution.HeLa cells were treated by staurosporine(1 uM) for 3 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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