F10, also known as Coagulation factor X, belongs to the peptidase S1 family that is synthesized as a 488 amino acid (aa) with a signal peptide and a pro region (residues 1-40). Both the intrinsic and extrinsic pathways activate Factor X to Xa, which consists of light (residues 41-179) and heavy (residues 235-488) chains linked by a disulfide bond. Coagulation factor X is initially synthesized in the liver. The two chains are formed from a single-chain precursor by the excision of two Arg residues and are held together by 1 or more disulfide bonds. Forms a heterodimer with SERPINA5. F10 is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
Product Name:
Coagulation Factor X/F10 Recombinant Protein (RPES3430)
Product Code:
RPES3430
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Coagulation factor 10,coagulation factor X,FX,FXA
Accession:
P00742
Sequence:
Asp22-Phe96
Fusion tag:
C-Fc
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Coagulation Factor X is produced by our Mammalian expression system and the target gene encoding Asn32-Lys488 is expressed with a Fc tag at the C-terminus.
Purity:
> 85% as determined by reducing SDS-PAGE.
Mol Mass:
78.2 kDa
AP Mol Mass:
94 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM MES,150mM NaCl,0.2mM CaCl2,pH5.5.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
