Lipid Peroxidation Assays
Quantify oxidative damage to lipids through MDA, TBARS and lipid peroxide, with kits matched to cell, tissue, serum and plant samples.
Malondialdehyde, the most widely reported end product of lipid peroxidation, with kits for cell and plant samples.
Thiobarbituric acid reactive substances in colorimetric and fluorometric formats.
Direct measurement of lipid hydroperoxides (LPO) by colorimetric or fluorometric read-out.
Reactive oxygen species attack the polyunsaturated fatty acids in cell membranes, setting off a chain reaction that produces lipid hydroperoxides and breaks down to aldehydes such as MDA. Lipid peroxidation is therefore a direct marker of oxidative damage, and it is the defining feature of ferroptosis.
In the TBARS assay, MDA reacts with thiobarbituric acid on heating to give a pink product that is read by absorbance or fluorescence.
MDA is one specific product of lipid peroxidation. TBARS measures everything in the sample that reacts with thiobarbituric acid, of which MDA is the main component.
A dedicated MDA kit for cell samples is available. Separate kits are matched to plant samples and to serum and tissue.
Fluorometric kits are more sensitive and suit samples with low levels of peroxidation.