Inhibitor Screening Assays
Ready-to-run plate-reader kits for testing candidate enzyme inhibitors, with the enzyme, substrate and controls supplied.
A fluorometric kit for screening inhibitors of angiotensin-converting enzyme 2.
Screen inhibitors of starch digestion for metabolic and food research.
Monoamine oxidase, nitric oxide synthase and arginase inhibitor kits are in high-throughput screening.
Each kit supplies an enzyme and a substrate that generates a colour or fluorescence signal. A test compound that inhibits the enzyme reduces that signal, so potency can be ranked across a plate and expressed as percent inhibition or IC50.
Your test compounds and a microplate reader. Enzyme, substrate, buffer and control inhibitor requirements are listed in each protocol.
Yes. Run a dilution series of the compound and fit percent inhibition against concentration.
Yes. See high-throughput screening and enzyme activity for further kits.