TBARS Assay Kit (BA0060)
- SKU:
- BA0060
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Sample Type:
- Serum, Plasma, Urine, Saliva
- Research Area:
- Lipid Metabolism
- Oxidative Stress
- Lipid Peroxidation
Frequently bought together:
Description
TBARS Assay Kit - Information
Assay Genie's TBARS assay is based on the reaction of TBARS with thiobarbituric acid (TBA) to form a pink colored product. The color intensity at 535nm or fluorescence intensity at (λex/em = 560nm/585nm) is directly proportional to TBARS concentration in the sample.Applications
For quantitative determination of lipid peroxides (thiobarbituric acid reactive substances, TBARS) and evaluation of drug effects on lipid peroxidation.TBARS Assay Kit - Key Features
Sensitive and accurate. Linear detection range: colorimetric assay 1 -30 , fluorometric assay 0.1 - 1.5 MDA. TBARS Assay Kit - Data Sheet | |
| Kit Includes | TBA Reagent: 25 mL Standard: 6 M MDA 10% Trichloroacetic acid (TCA): 25 mL |
| Kit Requires | Pipetting devices, centrifuge tubes, centrifuge, clear flat-bottom uncoated 96-well plates, optical density or fluorescence plate readers, sonicator, water-bath or heat block. |
| Method of Detection | OD535nm, or FL560/585nm |
| Detection Limit | 0.1 |
| Samples | Serum, plasma, urine, saliva etc |
| Species | All |
| Protocol Length | 80 min |
| Size | 100 tests |
| Storage | Store all components at -20 °C |
| Shelf Life | 6 months |
More Details
Oxidative attack of essential cell components by reactive oxygen species has been associated with several human diseases, such as atherosclerosis, cardiovascular diseases, diabetes, liver disorders, and inflammatory rheumatic diseases. THIOBARBITURIC ACID REACTIVE SUBSTANCES (TBARS) are low-molecular-weight end products (mainly malondialdehyde, MDA) that are formed during the decomposition of lipid peroxidation products. Increased levels of TBARS have been demonstrated in these diseases. Simple, direct and accurate assays for TBARS find wide applications in research and drug discovery.Contact Us for a Quote!
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