Human Multiplex Cytokine ELISA Panels

The ELISA Genie, Human Cytokine multiplex assays utilizes multiple bead populations differentiated by size and different levels of fluorescence intensity. With multiple sizes of beads and multiple levels of fluorescence intensity in each bead size, the GeniePlex technology can measure up to 24 analytes simultaneously using as little as 15μL.

The bead populations in the reaction are determined by a flow cytometer equipped with either a single 488nm laser or dual 488nm and 633/640nm lasers. The maximum emission of the bead classification dye is at 700 nm.

Features & Benefits

  • Analyze: Multiplex 2-24 analytes in a single sample!
  • Fast: 2 hour protocol!
  • Sensitive: Measure as low as <10 pg/ml of each analyte
  • Dynamic: Lower limit < 20 pg/mL | Upper limit > 5,000 pg/mL
  • High Precision Intra-assay CV: < 10% | Inter-assay CV: < 20%
  • Low Volumes: Use as little as 15ul of sample
  • Validated: All assays fully tested for cross-reactivity in our lab

Data Analysis

Human Multiplex Cytokine ELISA Panels

Product Code Panel Name Number of Analytes Analytes

Human T Helper Cytokine 3-Plex Immunoassay Panel 1

3

IL-9, IL-10, and TNFα

Human T Helper Cytokine 3-Plex Immunoassay Panel 2

3

IL-6, IL-10, and TNFα

Human T Helper Cytokine 3-Plex Immunoassay Panel 3

3

IFNγ, IL-10, and TNFα

Human T Helper Cytokine 4-Plex Immunoassay Panel 1

4

IFNγ, IL-4, IL-6, TNFα

Human T Helper Cytokine 4-Plex Immunoassay Panel 2

4

IFNγ, IL-4, IL-10, TNFα

Human T Helper Cytokine 4-Plex Immunoassay Panel 3

4

IFNγ, IL-10, TNFα, TNFβ

Human Th1/Th2 5-Plex Immunoassay Panel 1

5

IFNγ, IL-2, IL-6, IL-10, TNFα

Human Th1/Th2 5-Plex Immunoassay Panel 2

5

IFNγ, IL-4, IL-6, IL-10, TNFα

Human Th1/Th2 5-Plex Immunoassay Panel 3

5

IFNγ, IL-4, IL-5, IL-10, TNFα

Human Th1/Th2 6-Plex Immunoassay Panel 1

6

IFNγ, IL-2, IL-4, IL-6, IL-10, TNFα

Human Th1/Th2 6-Plex Immunoassay Panel 2

6

IFNγ, IL-2, IL-4, IL-5, IL-10, TNFα

Human Th1/Th2 7-Plex Immunoassay Panel

7

IFNγ, IL-2, IL-4, IL-5, IL-6, IL-10, TNFα

Human Th1/Th2 10-plex

10

IFNγ, IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, TNFα

Human Th1/Th2 11-plex

11

GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, TNFα

Human T Helper Cytokine 14-Plex Immunoassay Panel

14

GM-CSF, IFNγ, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL-17A, IL-21, IL-22 and TNFα

Human Th1/Th2/Th17 7-plex Immunoassay Panel

7

IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, TNFα

Human Th1/Th2/Th17 8-plex Immunoassay Panel 1

8

IFNγ, IL-2, IL-4, IL-6, IL-8, IL-10, IL-17A, TNFα

Human Th1/Th2/Th17 11-plex

11

IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-17A, TNFα, TNFβ

Human Th1/Th2/Th17 12-plex

12

IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-17A, TNFα, TNFβ

Human Th1/Th2/Th17 14-plex

14

IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IL-17F, IL-22, TNFα, TNFβ

Human Th1/Th2/Th17 18-plex

18

GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-9, IL-10, IL-12p70, IL-17A, IL-17F, IL-22, IL-33, TNFα, TNFβ, TSLP

Human Th1/Th2/Th17/Treg 10-Plex Immunoassay Panel

10

G-CSF, GM-CSF, IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, TNFα and TNFβ

Human Inflammation 6-Plex Immunoassay Panel

6

IL-1β, IL-6, IL-8, IL-10, IL-12p70, TNFα

Human Inflammation 7-Plex Immunoassay Panel

7

IL-1β, IL-1RA, IL-6, IL-8, IL-10, IL-12p70, TNFα

Human Inflammation 11-Plex Immunoassay Panel

11

IFNγ, IL-1α, IL-1β, IL-6, IL-8, IL-10, IL-12p70, IL-27, IP-10, MCP-1, TNFα

Human Inflammation 16-Plex Immunoassay Panel

16

IFNγ, IL-1α, IL-1β, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, IL-27, IL-31, IL-33, IP-10, MCP-1, MIP-1α, and TNFα

Human Inflammation 18-Plex Immunoassay Panel

18

IFNγ, IL-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, IL-27, IL-31, IL-33, IP-10, MCP-1, MIP-1α, MIP-1β, and TNFα

Human Chemokine 6-Plex Immunoassay Panel

6

Eotaxin, IL-8, RANTES, MCP-1, MCP-3, IP-10

Human Inflammatory Chemokine 7-Plex Immunoassay Panel 1

7

BCA-1, MCP-1, MIP-1α, MIP-3β, PARC, PF4, RANTES

Human Inflammatory Chemokine 7-Plex Immunoassay Panel 2

7

IP-10, I-TAC, MIP-1α, MIP-1β, 6Ckine, MDC, MIP-3β

Human CD8+ T Cell Related Cytokines, 14-plex Immunoassay Panel

14

GM-CSF, Granzyme B, IFNγ, IL-2, IL-4. IL-5, IL6, IL-10, IL-13, IL-15, MIP-1α, MIP-1β, sFasL, TNFα

Human CD8+ T Cell Related Cytokines, 16-plex Immunoassay Panel

16

GM-CSF, Granzyme B, IFNγ, IL-2, IL-4. IL-5, IL-6, IL-10, IL-13, IL-15, MIP-1α, MIP-1β, RANTES, sCD137, sFasL, TNFα

Kit Contents

  • Analyte-Specific antibody conjugated beads
  • Analyte-Specific biotinylated detection antibodies
  • Lyophilized Antigen Standards (One Vial)
  • Antibody diluent
  • 1x Streptavidin-PE (SAPE)
  • 10x Reading Buffer
  • 10x Wash Buffer
  • PCR 8-tube Strip
  • Filter Plate and Lid
  • Plate Seals

Human Multiplex Cytokine ELISA Panel Video Protocol