QuickStep ELISA Kits
QuickStep ELISA Kits — 90‑minute, 1‑wash assays
Get results faster without sacrificing quality. QuickStep ELISA kits run in just 90 minutes with a single wash step and only 50 µL of sample — saving up to two hours of bench time per plate.
Browse the QuickStep Range
Jump straight to the live catalog, or explore the wider fast-ELISA family.
All QuickStep Kits
Browse the complete, always-current QuickStep ELISA listing on the Assay Genie store.
View all QuickStep kitsPopular Kits
See a curated cross-section of our most-requested QuickStep ELISA kits.
View popular kitsWhy Choose QuickStep ELISA?
A faster, simpler immunoassay workflow validated for serum, plasma and other biological samples.
Fast
Complete your assay in just 90 minutes — save up to two hours of experimental time per plate.
Efficient
Only 50 µL of sample per well, conserving precious or low-volume specimens.
Easy
Simplified procedure with a single wash step and fewer handling errors.
Validated
Sensitive, specific and stable — validated in serum, plasma, urine and other fluids.
QuickStep ELISA Kits by Species
Browse the live, always-current QuickStep listing for your model — or use the filterable catalog further down.
Popular QuickStep ELISA Kits
A cross-section of our most-requested QuickStep kits, spanning inflammation, cardiac, hormone and metabolic biomarkers.
How QuickStep ELISA Works
Two assay principles cover proteins and small molecules alike.
Sandwich QuickStep ELISA
The plate is pre-coated with a capture antibody specific to the target. Sample and an HRP-linked detection antibody are added together; the antigen is captured between the two antibodies. After a single wash, substrate is added, the reaction is stopped, and optical density is read and compared against a standard curve. Best for larger proteins such as cytokines and biomarkers.
Learn about sandwich ELISACompetitive QuickStep ELISA
The plate is pre-coated with antigen. Sample antigen competes with the fixed plate antigen for binding to an HRP-linked antibody. After a single wash and substrate addition, signal is inversely proportional to sample concentration. Ideal for small molecules and hormones such as cortisol, estradiol, progesterone and testosterone.
Learn about competitive ELISAWhat Researchers Are Saying
Trusted by academic and industry labs worldwide.
“The kits were easy to use with an excellent datasheet which was clear and informative. I would recommend Assay Genie ELISA kits to experienced and inexperienced researchers alike.”
Michelle Ryan, Scientist, Marigot Ltd.“Our lab at Trinity College Dublin ordered several ELISA kits from Assay Genie. The kits were user-friendly and provided reliable results, and the technical support was excellent throughout.”
Javier Cuitavi, PhD, Trinity College DublinWhy Choose Assay Genie
Fast Delivery
7–10 business day lead time after manufacture.
Highly Cited
Thousands of citations across leading scientific journals.
PhD-Level Support
Our team is trained to a PhD level to support your assay questions.
Frequently Asked Questions
How fast is the QuickStep ELISA protocol?
QuickStep ELISA kits use a streamlined 90-minute protocol with a single wash step, saving up to two hours of hands-on time compared with conventional multi-wash sandwich ELISAs while maintaining sensitivity and specificity.
How much sample does a QuickStep ELISA need?
Each assay requires just 50 uL of sample, making QuickStep kits ideal for precious or low-volume specimens such as serum, plasma and other biological fluids.
What is the difference between QuickStep sandwich and competitive ELISA kits?
Sandwich QuickStep kits use a pre-coated capture antibody plus an HRP-linked detection antibody and are used for larger proteins. Competitive QuickStep kits use a pre-coated antigen and are used for small molecules and hormones such as cortisol, estradiol and testosterone, where the sample analyte competes with fixed antigen for the HRP-linked antibody.
Which species do QuickStep ELISA kits cover?
QuickStep ELISA kits are available for human, mouse and rat targets, plus cross-reactive hormone kits validated in bovine, porcine, canine, monkey, chicken, sheep, rabbit and horse samples.
What sample types are QuickStep ELISA kits validated for?
QuickStep kits are validated for use in serum, plasma, urine, saliva and other biological fluids. Validated matrices are listed on each product datasheet.
Are QuickStep ELISA kits for research use only?
Yes. All Assay Genie QuickStep ELISA kits are intended strictly for research use only (RUO) and are not for diagnostic or therapeutic use.
Got a Question?
Tell our PhD-level technical team which analyte and species you're working with, and we'll help you choose the right QuickStep ELISA kit.
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