Human IGF-1 (Insulin-like Growth Factor 1) Quickstep ELISA Kit
The QuickStep Human IGF-1 (Insulin-like Growth Factor 1) ELISA Kit is a cutting-edge tool designed for the precise measurement of IGF-1 levels in human samples including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers highly accurate and consistent results, making it an invaluable asset for various research endeavors.IGF-1 is a key hormone that regulates growth and development, playing a critical role in numerous physiological processes such as cell growth, metabolism, and tissue repair. Its dysregulation has been linked to various health conditions including growth disorders, diabetes, and cancer, highlighting the significance of IGF-1 as a biomarker for disease diagnosis and therapeutic research.Empower your research with the QuickStep Human IGF-1 ELISA Kit, a reliable solution for studying the intricate mechanisms of IGF-1 signaling and its implications in human health and disease.
Product Name:
Human IGF-1 (Insulin-like Growth Factor 1) Quickstep ELISA Kit
SKU:
QSES026
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
1 h 30 min
Sensitivity:
1.01 ng/mL
Detection range:
3.12-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
