Human IGF-1 (Insulin-Like Growth Factor 1) ELISA Kit
The Human IGF-1 (Insulin-like Growth Factor 1) ELISA Kit is specifically designed for the precise measurement of IGF-1 levels in human serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a diverse range of research applications.IGF-1, a key growth factor, plays a critical role in promoting cell growth, differentiation, and metabolism. It is closely associated with various physiological processes, including tissue repair, muscle growth, and bone development. Dysregulation of IGF-1 levels has been linked to a variety of disorders, such as diabetes, cancer, and growth deficiencies, making it a valuable biomarker for understanding disease mechanisms and potential therapeutic interventions.Overall, the Human IGF-1 ELISA Kit provides researchers with a powerful tool for investigating the role of IGF-1 in health and disease, offering valuable insights into cellular processes and opening new avenues for innovative research and treatment strategies.
Product Name:
Human IGF-1 (Insulin-Like Growth Factor 1) ELISA Kit
SKU:
HUES01372
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
