The QuickStep Human IL-6 (Interleukin-6) ELISA Kit is specifically designed for the accurate and sensitive detection of IL-6 levels in human serum, plasma, and cell culture supernatants. This kit offers high specificity and sensitivity, providing reliable and reproducible results for a variety of research applications.IL-6 is a key cytokine involved in inflammation and immune response, playing a critical role in various diseases including autoimmune disorders, cancer, and infectious diseases. Monitoring IL-6 levels can provide valuable insights into disease progression and response to treatment, making it a valuable biomarker for research and clinical studies. With the QuickStep Human IL-6 ELISA Kit, researchers can accurately measure IL-6 levels in human samples, enabling further understanding of the role of this cytokine in different disease conditions and potential therapeutic interventions. This kit is easy to use, offering a convenient and efficient solution for studying IL-6 levels in various research settings.
Product Name:
Human IL-6 Quickstep ELISA Kit
SKU:
QSES015
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
1 h 30 min
Sensitivity:
1.02 pg/mL
Detection range:
3.13-200 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
