Lipid Metabolism Assays

Lipid Metabolism - Overview

Lipid metabolism is the process by which fats are broken down and used by the body to generate energy or to synthesise new structural and functional lipids. Lipid metabolism is a complex and tightly regulated process that is necessary for maintaining health and preventing disease. Lipids are an essential part of the diet and are required for a number of crucial functions in the body. Lipid metabolism occurs in a number of different ways, depending on the type of lipid being metabolised such as cholesterol, phospholipids etc.

Beta-Oxidation: The Energy Powerhouse

As the primary method of fatty acid degradation, beta-oxidation is a metabolic process that breaks down fatty acids within the mitochondria to generate energy-rich molecules, such as ATP. This process is pivotal, particularly during periods of fasting or prolonged exercise when glucose supply is limited. By breaking down stored fatty acids, beta-oxidation ensures a constant energy supply, supporting our body's continuous functioning.

Lipogenesis: The Art of Building Fats

Lipogenesis is the process by which our bodies synthesize fatty acids. This metabolic pathway, primarily occurring in the liver and adipose tissue, takes glucose and other small molecules and assembles them into fatty acids. These newly synthesized fatty acids can then be stored as triglycerides for future energy use, signifying the critical role of lipogenesis in energy balance and storage.

Cholesterol Metabolism: Balancing the Scales

Cholesterol metabolism is another vital aspect of lipid metabolism. Cholesterol, while often villainized, is an essential component of cell membranes and a precursor to steroid hormones and bile acids. Our bodies must carefully balance cholesterol synthesis and excretion to maintain optimal cellular and physiological function. Too much or too little cholesterol can lead to health problems, highlighting the importance of regulated cholesterol metabolism.

Phospholipid Metabolism: Ensuring Cellular Integrity

Phospholipids are unique lipids that form the fundamental structure of cell membranes. Phospholipid metabolism involves the synthesis and degradation of these crucial molecules. This metabolic process ensures cell membrane integrity, enabling cells to function effectively while also playing a role in cell signaling and lipid transport.

Eicosanoid Metabolism: The Signaling Maestros

Eicosanoids are signaling molecules derived from fatty acids that play crucial roles in immune response and inflammation. The metabolism of eicosanoids involves the conversion of essential fatty acids into these biologically active compounds, which then regulate various physiological processes.

Lipid Metabolism Assays

Product CodeProduct NameInstrumentSample Type
MAES0131Acetylcholinesterase Activity Assay KitMicroplate Reader, Biochemistry AnalyserSerum, Plasma, Cells, Culture Supernatent, Tissue
BA0076Adipolysis Assay KitSpectrophotometerCulture Media
MAES0124Biuret Protein Assay KitMicroplate ReaderAnimal Tissue, Cells
MAES0125Biuret Protein Assay KitSpectrophotometerSerum, Animal Tissue, Cells
MAES0126Bradford Protein Assay KitMicroplate Reader, Biochemistry AnalyserSerum, Plasma, Tissue Homogenate, Cells supernatent
MAES0127Bradford Protein Assay KitSpectrophotometerTissues and Cells
MAES0139Chlorine Assay KitMicroplate ReaderTissues, Cells
BA0064Cholesterol Assay KitSpectrophotometer, Fluorescence Microplate ReaderSerum, Plasma, etc.
BA0084Cholesterol Assay KitSpectrophotometerSerum, Plasma, etc.
BA0021Cholesterol Assay KitFluorescence Microplate ReaderAdherent Cells
MAES0138Creatinine AssaySpectrophotometer, Biochemistry AnalyserSerum
BR00001Fatty Acid Oxidation AssayMicroplate ReaderCell and tissues
BA0184Fatty Acid Uptake Assay KitFluorescence Microplate ReaderAdipocytes and other fatty acid transporting cells
BA0101Free Fatty Acid Measurement KitSpectrophotometer, Fluorescence Microplate ReaderSerum, Plasma, Urine, Saliva, Milk, cell culture, Food, etc.
BA0066HDL and LDL/VLDL Assay KitSpectrophotometer, Fluorescence Microplate ReaderSerum
BA0119HDL and LDL/VLDL Assay KitSpectrophotometerserum
BA0089Isocitrate Assay KitSpectrophotometerFood, beverages, Biological samples
BA0038Isocitrate Dehydrogenase Assay KitSpectrophotometerPlasma, Serum, Tissue, Culture Media
BA0052Lipase Activity Assay KitSpectrophotometerSerum, Plasma, Saliva, Urine, etc.
MAES0132Lipid Peroxide (LPO) Assay KitSpectrophotometerSerum, Plasma, Cells, Culture Supernatent, Tissue
MAES0122Magnesium Assay KitFluorescence Microplate ReaderSerum, Plasma, Animal Tissue
MAES0123Magnesium Assay KitMicroplate ReaderSerum, Plasma, Tissue
BA0144Phospholipase D Assay KitSpectrophotmeter, Fluorescence Microplate PlateBiological
BA0143Phospholipid Assay KitSpectrophotometerBiological
MAES0133Proline (PRO) Assay KitMicroplate Reader, Biochemistry AnalyserSerum, Plasma, Cells, Culture Supernatent, Tissue
MAES0121Sucrose Assay KitFluorescence Microplate ReaderSerum, Plasma, Animal Tissue, Cells
BA0060TBARS AssaySpectrophotmeter, Fluorescence Microplate PlateSerum, Plasma, Urine, Saliva, etc
MAES0134Total Bile Acid (TBA) Assay KitSpectrophotometerSerum, Plasma, Cells, Culture Supernatent, Tissue
MAES0135Total Bile Acid (TBA) Assay KitMicroplate Reader, Biochemistry AnalyserSerum, Plasma, Cells, Culture Supernatent
MAES0128Total Carbonyl Assay KitMicroplate ReaderSerum, Plasma, Tissue,Cells,Cell Culture Supernatent
MAES0129Total Carbonyl Assay KitSpectrophotometerSerum, Plasma, Hydrothorax, Animal Tissue, Cells
BA0152Triglyceride Assay KitSpectrophotmeterSerum, Plasma, etc.
MAES0136Urea (BUN) Assay KitMicroplate ReaderSerum, Plasma, Cells, Tissues
MAES0137Urea (BUN) Assay KitSpectrophotometerSerum, Plasma, Cells, Tissues