Triglyceride Assay Kit
Triglyceride , also known as Triacyltriglyceride or Triacyl-glyceride, is the main constituent in vegetable oil and animal fats. Triglycerides play an important role as energy sources and transporters of dietary fat. In the human body, high levels of triglycerides in the bloodstream have been linked to atherosclerosis, heart disease and pancreatitis. Simple, direct and automation-ready procedures for measuring triglyceride concentrations find wide applications in research and drug discovery.Assay Genie's triglyceride assay uses a single working reagent that combines triglyceride hydrolysis and glycerol determination in one step, in which a dye reagent is oxidized to form a colored product. The color intensity at 570nm is directly proportional to triglyceride concentration in the sample.
ApplicationsFor quantitative determination of triglyceride and evaluation of drug effects on triglyceride metabolism.
Triglyceride Assay Kit - Key Features
- Sensitive and accurate. Use as little as 10 uL samples. Linear detection range 0.01 to 1.0 mmol/L (0.88 to 88.5 mg/dL) triglyceride.
- Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature, compatible for HTS assays.
- Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.
Triglyceride Assay Kit Information:
|Kit Includes:||Assay Buffer: 24 mL, ATP: 250 mL, Dye Reagent: 220 mL, Enzyme Mix: 500 mL, Lipase: 1000 mL, Standard: 100 mL (equivalent to 100 mmol/L Triglyceride)|
|Kit Requires:||Pipeting devices, centrifuge tubes, clear flat bottom 96-well plates and plate reader.|
|Method of Detection:||OD570nm|
|Detection Limit:||0.01 mmol/L (0.88 mg/dL)|
|Samples:||Serum, plasma etc|
|Protocol Length:||30 min|
|Storage:||Store Assay Buffer at 4°C and other reagents at -20°C.|
|Shelf Life:||12 months|