KO Validated Antibodies

Figure 1: Immunohistochemistry of paraffin-embedded human colon using KO validated FASN antibody (CAB0462) at dilution of 1:100 (40x lens).

Figure 2: Immunofluorescence analysis of HeLa cells using KO validated KRT8 antibody (CAB1024) at dilution of 1:100. Blue: DAPI for nuclear staining.

What are KO Validated Antibodies?

KO validation is a means by which antibody specificity is confirmed by testing antibodies in a knockout (KO) cell line.

Using KO cell lines (cells with or no expression/reduced expression of precise target proteins) is the new gold standard to validate the specificity of antibodies.

If a signal is detected from a KO cell line, then the antibody is not specific enough. This is due to the antibody binding to a similar protein within the sample. Antibodies with high specificity on the other hand will not produce any signal.

KO validated antibodies must be shown to be specific, selective, and reproducible.

Why is it important to validate antibodies?

Antibodies are one of the most commonly used research tools. These proteins are used routinely in experiments such as Western blot, ELISA, immunostaining (immunohistochemistry (IHC) and immunocytochemistry (ICC)), immunofluorescence, and flow cytometry among others.

For the findings of these experimental techniques to be reliable they must have an internally high standard of accuracy and precision. This is why there is a need for highly specific antibodies that can ensure reproducibility of results.

How do we validate our Knockout Antibodies?

We have created hundreds of CRISPR Knockout cell lines by introducing frame-shift mutations into the genomic DNA of target cells using sgRNA/CAS9.

The CRISPR knockout cells were lysed and Western Blotting was performed using Wild-Type (WT) cells as a control.

The Western Blots were then probed using our premium antibodies. Visualization of decreased protein expression was indicative of highly specific antibodies (Knockout Validated Antibodies).

Figure 3: Schematic representation of how KO validated antibodies are created using CRISPR technology.

Top Validated Antibody targets

SKU Code Product

BCL-10 Knockout Validated Polyclonal Antibody

CAPG Knockout Validated Polyclonal Antibody

KRT8 Knockout Validated Polyclonal Antibody

GIT1 Knockout Validated Polyclonal Antibody

EEF1B2 Knockout Validated Polyclonal Antibody

AK2 Knockout Validated Polyclonal Antibody

CDK8 Knockout Validated Polyclonal Antibody

EGFR Knockout Validated Polyclonal Antibody

NF2 Knockout Validated Polyclonal Antibody

Related Tools for Research